Stimulus-coupled taurine efflux from cerebellar neuronal cultures: on the roles of Ca++ and Na+

R A Philibert; K L Rogers; G R Dutton

doi:10.1002/jnr.490220209

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Stimulus-coupled taurine efflux from cerebellar neuronal cultures: on the roles of Ca++ and Na+

Journal article

Peer reviewed

Stimulus-coupled taurine efflux from cerebellar neuronal cultures: on the roles of Ca++ and Na+

R A Philibert, K L Rogers and G R Dutton

Journal of neuroscience research, Vol.22(2), pp.167-171

02/1989

DOI: 10.1002/jnr.490220209

PMID: 2468785

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Abstract

Primary cultures of cerebellar neurons obtained from 7-9-day-old rats and grown 7-9 days in vitro (DIV) were used to study the effects of Na+ and Ca++ on K+-evoked taurine release. These cultures, made up largely of granule neurons (90%) and inhibitory interneurons (5-7%), produced a dose-dependent, depolarization-evoked taurine release that was Ca++-dependent at 40 mM K+, and Ca++-independent at K+ concentrations above 40 mM. The dihydropyridine Ca++ channel agonist BAY K 8644 (1 microM) augmented 30 mM K+-evoked release, while the antagonist nifedipine (5 microM) abolished both the BAY K 8644- and K+-enhanced release. Depolarization with the Na+ channel agonist veratridine (50 microM) stimulated taurine efflux, which was completely blocked by pretreatment with tetrodotoxin (2 microM). However, 50 mM K+-evoked taurine release was not affected by tetrodotoxin pretreatment. Substitution of choline Cl for NaCl partially antagonized 50 mM K+-evoked release, and by itself, the Na+ ionophore monensin (50 microM) stimulated release. These results suggest that both K+-evoked and basal taurine release from primary cerebellar neuronal cultures are sensitive to the levels of both intracellular and extracellular Na+ and Ca++. In contrast to previous findings using cerebellar astrocytes, neuronal L-type Ca++ channels, but not voltage-dependent Na+ channels, also appear to be necessary. The implications of these results on taurine's status as a putative neurotransmitter are discussed.

Sodium - pharmacology

Nifedipine - pharmacology

Calcium - pharmacology

Cerebellum - metabolism

Cells, Cultured

3-Pyridinecarboxylic acid, 1,4-dihydro-2,6-dimethyl-5-nitro-4-(2-(trifluoromethyl)phenyl)-, Methyl ester - pharmacology

Monensin - pharmacology

Rats

Veratridine - pharmacology

Animals

Choline - pharmacology

Neurons - metabolism

Kinetics

Neurons - drug effects

Taurine - metabolism

Details

Title: Subtitle: Stimulus-coupled taurine efflux from cerebellar neuronal cultures: on the roles of Ca++ and Na+
Creators: R A Philibert - Department of Pharmacology, College of Medicine, University of Iowa, Iowa City 52242
K L Rogers
G R Dutton
Resource Type: Journal article
Publication Details: Journal of neuroscience research, Vol.22(2), pp.167-171
DOI: 10.1002/jnr.490220209
PMID: 2468785
NLM abbreviation: J Neurosci Res
ISSN: 0360-4012
eISSN: 1097-4547
Publisher: United States
Grant note: MH 14620 / NIMH NIH HHS NS 20632 / NINDS NIH HHS GMO7337 / NIGMS NIH HHS
Language: English
Date published: 02/1989
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Psychiatry; Iowa Neuroscience Institute; Neuroscience and Pharmacology
Record Identifier: 9984004093602771

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