Journal article
Stomatocytosis Is Absent in “Stomatin”-Deficient Murine Red Blood Cells
Blood, Vol.93(7), pp.2404-2410
04/01/1999
DOI: 10.1182/blood.V93.7.2404.407k13_2404_2410
PMID: 10090952
Abstract
To examine the relationship between erythrocyte membrane protein 7.2b deficiency and the hemolytic anemia of human hereditary stomatocytosis, we created 7.2b knock-out mice by standard gene targeting approaches. Immunoblots showed that homozygous knock-out mice completely lacked erythrocyte protein 7.2b. Despite the absence of protein 7.2b, there was no hemolytic anemia and mouse red blood cells (RBCs) were normal in morphology, cell indices, hydration status, monovalent cation content, and ability to translocate lipids. The absence of the phenotype of hereditary stomatocytosis implies that protein 7.2b deficiency plays no direct role in the etiology of this disorder and casts doubt on the previously proposed role of this protein as a mediator of cation transport in RBC.
Details
- Title: Subtitle
- Stomatocytosis Is Absent in “Stomatin”-Deficient Murine Red Blood Cells
- Creators
- Yiwen Zhu - From the Department of Pediatrics, University of California San Francisco, San Francisco, CA; the Department of Pediatrics, Yale University School of Medicine, New Haven, CT; the Lawrence Berkeley National Laboratory, Berkeley, CA; and the Childrens Hospital Oakland Research Institute, Oakland, CAChris Paszty - From the Department of Pediatrics, University of California San Francisco, San Francisco, CA; the Department of Pediatrics, Yale University School of Medicine, New Haven, CT; the Lawrence Berkeley National Laboratory, Berkeley, CA; and the Childrens Hospital Oakland Research Institute, Oakland, CATikva Turetsky - From the Department of Pediatrics, University of California San Francisco, San Francisco, CA; the Department of Pediatrics, Yale University School of Medicine, New Haven, CT; the Lawrence Berkeley National Laboratory, Berkeley, CA; and the Childrens Hospital Oakland Research Institute, Oakland, CASusan Tsai - From the Department of Pediatrics, University of California San Francisco, San Francisco, CA; the Department of Pediatrics, Yale University School of Medicine, New Haven, CT; the Lawrence Berkeley National Laboratory, Berkeley, CA; and the Childrens Hospital Oakland Research Institute, Oakland, CAFrans A Kuypers - From the Department of Pediatrics, University of California San Francisco, San Francisco, CA; the Department of Pediatrics, Yale University School of Medicine, New Haven, CT; the Lawrence Berkeley National Laboratory, Berkeley, CA; and the Childrens Hospital Oakland Research Institute, Oakland, CAGloria Lee - From the Department of Pediatrics, University of California San Francisco, San Francisco, CA; the Department of Pediatrics, Yale University School of Medicine, New Haven, CT; the Lawrence Berkeley National Laboratory, Berkeley, CA; and the Childrens Hospital Oakland Research Institute, Oakland, CAPhilip Cooper - From the Department of Pediatrics, University of California San Francisco, San Francisco, CA; the Department of Pediatrics, Yale University School of Medicine, New Haven, CT; the Lawrence Berkeley National Laboratory, Berkeley, CA; and the Childrens Hospital Oakland Research Institute, Oakland, CAPatrick G Gallagher - From the Department of Pediatrics, University of California San Francisco, San Francisco, CA; the Department of Pediatrics, Yale University School of Medicine, New Haven, CT; the Lawrence Berkeley National Laboratory, Berkeley, CA; and the Childrens Hospital Oakland Research Institute, Oakland, CAMary E Stevens - From the Department of Pediatrics, University of California San Francisco, San Francisco, CA; the Department of Pediatrics, Yale University School of Medicine, New Haven, CT; the Lawrence Berkeley National Laboratory, Berkeley, CA; and the Childrens Hospital Oakland Research Institute, Oakland, CAEdward Rubin - From the Department of Pediatrics, University of California San Francisco, San Francisco, CA; the Department of Pediatrics, Yale University School of Medicine, New Haven, CT; the Lawrence Berkeley National Laboratory, Berkeley, CA; and the Childrens Hospital Oakland Research Institute, Oakland, CANarla Mohandas - From the Department of Pediatrics, University of California San Francisco, San Francisco, CA; the Department of Pediatrics, Yale University School of Medicine, New Haven, CT; the Lawrence Berkeley National Laboratory, Berkeley, CA; and the Childrens Hospital Oakland Research Institute, Oakland, CAWilliam C Mentzer - From the Department of Pediatrics, University of California San Francisco, San Francisco, CA; the Department of Pediatrics, Yale University School of Medicine, New Haven, CT; the Lawrence Berkeley National Laboratory, Berkeley, CA; and the Childrens Hospital Oakland Research Institute, Oakland, CA
- Resource Type
- Journal article
- Publication Details
- Blood, Vol.93(7), pp.2404-2410
- DOI
- 10.1182/blood.V93.7.2404.407k13_2404_2410
- PMID
- 10090952
- ISSN
- 0006-4971
- eISSN
- 1528-0020
- Language
- English
- Date published
- 04/01/1999
- Academic Unit
- Iowa Neuroscience Institute; Immunology; Internal Medicine
- Record Identifier
- 9984070565702771
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