Journal article
Strains and clones of Trypanosoma cruzi differ in their expression of a surface antigen identified by a monoclonal antibody
Molecular and biochemical parasitology, Vol.11(C), pp.81-89
1984
DOI: 10.1016/0166-6851(84)90056-2
PMID: 6205268
Abstract
Four
Trypanosoma cruzi strains and 50 clones derived from a total of 10 strains were assayed with a monoclonal antibody, WIC 29.26 Ab, for expression of an epitope previously demonstrated to be on the carbohydrate portion of a 72 000 Da surface glycoprotein of Y strain epimastigotes. WIC 29.26 Ab bound to only 2 of the 4 strains and 23 of the 50 clones tested. A group of 10 cloned isolates from one strain contained both reactive and non-reactive clones. Competitive inhibition studies with soluble extracts of the reactive and non-reactive isolates suggested that in addition to being absent from the surface membrane, the antigenic determinant is not synthesized by the non-reactive parasites. These data indicate that the epitope recognized by WIC 29.26 Ab is not present on all
T. cruzi epimastigotes, and provide the first demonstration of clone-specific differences in a parasite antigen detected by a monoclonal antibody. No correlation was found between the reactivity of isolates with WIC 29.26 Ab and the previously investigated parameters of growth rate and modal volume.
Details
- Title: Subtitle
- Strains and clones of Trypanosoma cruzi differ in their expression of a surface antigen identified by a monoclonal antibody
- Creators
- Louis V Kirchhoff - National Institutes of HealthJuan C Engel - National Institutes of HealthJames A Dvorak - National Institutes of HealthAlan Sher - National Institutes of Health
- Resource Type
- Journal article
- Publication Details
- Molecular and biochemical parasitology, Vol.11(C), pp.81-89
- Publisher
- Elsevier B.V
- DOI
- 10.1016/0166-6851(84)90056-2
- PMID
- 6205268
- ISSN
- 0166-6851
- eISSN
- 1872-9428
- Language
- English
- Date published
- 1984
- Academic Unit
- Psychiatry; Infectious Diseases; Epidemiology; Internal Medicine
- Record Identifier
- 9984280865202771
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