Journal article
Streptococcus mutans glucan-binding protein-A affects Streptococcus gordonii biofilm architecture
FEMS microbiology letters, Vol.267(1), pp.80-88
02/01/2007
DOI: 10.1111/j.1574-6968.2006.00557.x
PMCID: PMC1780135
PMID: 17166223
Abstract
Abstract
The glucan-binding protein-A (GbpA) of Streptococcus mutans has been shown to contribute to the architecture of glucan-dependent biofilms formed by this species and influence virulence in a rat model. As S. mutans synthesizes multiple glucosyltransferases and nonglucosyltransferase glucan-binding proteins (GBPs), it is possible that there is functional redundancy that overshadows the full extent of GbpA contributions to S. mutans biology. Glucan-associated properties such as adhesion, aggregation, and biofilm formation were examined independently of other S. mutans GBPs by cloning the gbpA gene into a heterologous host, Streptococcus gordonii, and derivatives with altered or diminished glucosyltransferase activity. The presence of GbpA did not alter dextran-dependent aggregation nor the initial sucrose-dependent adhesion of S. gordonii. However, expression of GbpA altered the biofilm formed by wild-type S. gordonii as well as the biofilm formed by strain CH107 that produced primarily α-1,6-linked glucan. Expression of gbpA did not alter the biofilm formed by strain DS512, which produced significantly lower quantities of parental glucan. These data are consistent with a role for GbpA in facilitating the development of biofilms that harbor taller microcolonies via binding to α-1,6-linkages within glucan. The magnitude of the GbpA effect appears to be dependent on the quantity and linkage of available glucan.
Details
- Title: Subtitle
- Streptococcus mutans glucan-binding protein-A affects Streptococcus gordonii biofilm architecture
- Creators
- Jeffrey A Banas - Center for Immunology and Microbial Disease, Albany Medical College, Albany, New YorkTracey L Fountain - Center for Immunology and Microbial Disease, Albany Medical College, Albany, New YorkJoseph E Mazurkiewicz - Center for Neuroscience and Neuropharmacology, Albany Medical College, Albany, New YorkKeer Sun - Center for Immunology and Microbial Disease, Albany Medical College, Albany, New YorkM. Margaret Vickerman - Department of Periodontics and Endodontics, State University of New York at Buffalo, South Campus, Buffalo, New York
- Resource Type
- Journal article
- Publication Details
- FEMS microbiology letters, Vol.267(1), pp.80-88
- Publisher
- Blackwell Publishing Ltd; Oxford, UK
- DOI
- 10.1111/j.1574-6968.2006.00557.x
- PMID
- 17166223
- PMCID
- PMC1780135
- ISSN
- 0378-1097
- eISSN
- 1574-6968
- Language
- English
- Date published
- 02/01/2007
- Academic Unit
- Pediatric Dentistry; Dental Research
- Record Identifier
- 9984065984702771
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