Streptococcus pyogenes causing toxic-shock-like syndrome and other invasive diseases: clonal diversity and pyrogenic exotoxin expression

James M Musser; Alan R Hauser; Michael H Kim; Patrick M Schlievert; Kimberlyn Nelson; Robert K Selander

doi:10.1073/pnas.88.7.2668

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Streptococcus pyogenes causing toxic-shock-like syndrome and other invasive diseases: clonal diversity and pyrogenic exotoxin expression

Journal article

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Streptococcus pyogenes causing toxic-shock-like syndrome and other invasive diseases: clonal diversity and pyrogenic exotoxin expression

James M Musser, Alan R Hauser, Michael H Kim, Patrick M Schlievert, Kimberlyn Nelson and Robert K Selander

Proceedings of the National Academy of Sciences - PNAS, Vol.88(7), pp.2668-2672

04/01/1991

DOI: 10.1073/pnas.88.7.2668

PMCID: PMC51299

PMID: 1672766

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Abstract

Genetic diversity and relationships among 108 isolates of the bacterium Streptococcus pyogenes recently recovered from patients in the United States with toxic-shock-like syndrome or other invasive diseases were estimated by multilocus enzyme electrophoresis. Thirty-three electrophoretic types (ETs), representing distinctive multilocus clonal genotypes, were identified, but nearly half the disease episodes, including more than two-thirds of the cases of toxic-shock-like syndrome, were caused by strains of two related clones (ET 1 and ET 2). These two clones were also represented by recent pathogenic European isolates. A previous report of a relatively high frequency of expression of exotoxin A among isolates recovered from toxic-shock-like syndrome patients in the United States was confirmed; and the demonstration of this association both within clones and among distantly related clones supports the hypothesis that exotoxin A is a causal factor in pathogenesis of this disease. Near identity of the nucleotide sequences of the exotoxin A structural gene of six isolates of five ETs in diverse phylogenetic lineages was interpreted as evidence that the gene has been horizontally distributed among clones, presumably by bacteriophage-mediated transfer.

Details

Title: Subtitle: Streptococcus pyogenes causing toxic-shock-like syndrome and other invasive diseases: clonal diversity and pyrogenic exotoxin expression
Creators: James M Musser - Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia 19104
Alan R Hauser - Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia 19104
Michael H Kim - Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia 19104
Patrick M Schlievert - Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia 19104
Kimberlyn Nelson - Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia 19104
Robert K Selander - Department of Pathology and Laboratory Medicine, Hospital of the University of Pennsylvania, Philadelphia 19104
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.88(7), pp.2668-2672
DOI: 10.1073/pnas.88.7.2668
PMID: 1672766
PMCID: PMC51299
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Language: English
Date published: 04/01/1991
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984001159602771

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