Streptococcus pyogenes pharyngitis: characterization of strains by multilocus enzyme genotype, M and T protein serotype, and pyrogenic exotoxin gene probing

James M Musser; Barry M Gray; Patrick M Schlievert; Michael E Pichichero

doi:10.1128/JCM.30.3.600-603.1992

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Streptococcus pyogenes pharyngitis: characterization of strains by multilocus enzyme genotype, M and T protein serotype, and pyrogenic exotoxin gene probing

Journal article

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Streptococcus pyogenes pharyngitis: characterization of strains by multilocus enzyme genotype, M and T protein serotype, and pyrogenic exotoxin gene probing

James M Musser, Barry M Gray, Patrick M Schlievert and Michael E Pichichero

Journal of clinical microbiology, Vol.30(3), pp.600-603

03/1992

DOI: 10.1128/JCM.30.3.600-603.1992

PMCID: PMC265117

PMID: 1551976

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Abstract

Multilocus enzyme electrophoresis, serological characterization of M and T proteins, and probing for pyrogenic exotoxin A and C genes were used to investigate the bacteriologic epidemiology of strains of Streptococcus pyogenes recovered primarily from patients with recurrent pharyngitis. A total of 164 strains recovered from individuals living in nine states of the United States was analyzed. Two-thirds of the patients in our sample were infected with the homologous strain following antibiotic therapy and presumably represented treatment failures, whereas the other one-third of the patients were infected with a heterologous strain after therapy and probably represented reinfections. Multilocus enzyme electrophoresis was as efficacious in strain discrimination as serologic typing techniques were and, in addition, successfully characterized all organisms that were serologically nontypeable. Two clones of S. pyogenes responsible for most of the episodes of toxic shock-like syndrome in the United States are geographically widespread, but they vary by locality in the frequency of their occurrence. Compared with a sample of strains cultured from patients whose pharyngeal infections were eliminated by antimicrobial therapy, these two clones were statistically overrepresented among organisms that cause recurrent pharyngitis.

Pharyngitis - microbiology

Streptococcal Infections - microbiology

United States - epidemiology

Bacterial Outer Membrane Proteins

Humans

Streptococcal Infections - epidemiology

Genotype

Antigens, Bacterial

Pharyngitis - epidemiology

Membrane Proteins

Streptococcus pyogenes - genetics

Epidemiologic Methods

Enzymes - genetics

Streptococcus pyogenes - enzymology

Exotoxins - genetics

Streptococcus pyogenes - classification

Bacterial Proteins - isolation & purification

Serotyping

Carrier Proteins

Details

Title: Subtitle: Streptococcus pyogenes pharyngitis: characterization of strains by multilocus enzyme genotype, M and T protein serotype, and pyrogenic exotoxin gene probing
Creators: James M Musser - Department of Pathology, Baylor College of Medicine, Houston, Texas 77030
Barry M Gray
Patrick M Schlievert
Michael E Pichichero
Resource Type: Journal article
Publication Details: Journal of clinical microbiology, Vol.30(3), pp.600-603
Publisher: United States
DOI: 10.1128/JCM.30.3.600-603.1992
PMID: 1551976
PMCID: PMC265117
ISSN: 0095-1137
eISSN: 1098-660X
Grant note: RR-05425 / NCRR NIH HHS
Language: English
Date published: 03/1992
Academic Unit: Microbiology and Immunology; Internal Medicine
Record Identifier: 9984001119902771

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