Journal article
Structural Basis for a Novel Interaction between the NS1 Protein Derived from the 1918 Influenza Virus and RIG-I
Structure (London), Vol.23(11), pp.2001-2010
11/03/2015
DOI: 10.1016/j.str.2015.08.007
PMCID: PMC4635043
PMID: 26365801
Abstract
The influenza non-structural protein 1 (NS1) plays a critical role in antagonizing the innate immune response to infection. One interaction that facilitates this function is between NS1 and RIG-I, one of the main sensors of influenza virus infection. While NS1 and RIG-I are known to interact, it is currently unclear whether this interaction is direct or if it is mediated by other biomolecules. Here we demonstrate a direct, strain-dependent interaction between the NS1 RNA binding domain (NS1 RBD) of the influenza A/Brevig Mission/1918 H1N1 (1918 H1N1) virus and the second caspase activation and recruitment domain of RIG-I. Solving the solution structure of the 1918 H1N1 NS1 RBD revealed features in a functionally novel region that may facilitate the observed interaction. The biophysical and structural data herein suggest a possible mechanism by which strain-specific differences in NS1 modulate influenza virulence.
Details
- Title: Subtitle
- Structural Basis for a Novel Interaction between the NS1 Protein Derived from the 1918 Influenza Virus and RIG-I
- Creators
- Alexander S. Jureka - University of Alabama at BirminghamAlex B. Kleinpeter - University of Alabama at BirminghamGabriel Cornilescu - University of Wisconsin–MadisonClaudia C. Cornilescu - University of Wisconsin–MadisonChad M. Petit - University of Alabama at Birmingham
- Resource Type
- Journal article
- Publication Details
- Structure (London), Vol.23(11), pp.2001-2010
- DOI
- 10.1016/j.str.2015.08.007
- PMID
- 26365801
- PMCID
- PMC4635043
- NLM abbreviation
- Structure
- ISSN
- 0969-2126
- eISSN
- 1878-4186
- Publisher
- Elsevier
- Number of pages
- 10
- Grant note
- IRG-60-001-53 / American Cancer Society 1P30 CA-13148 / NCI; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Cancer Institute (NCI) P41GM103399 / NIH; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA 1S10RR022994-01A1 / NCRR; United States Department of Health & Human Services; National Institutes of Health (NIH) - USA; NIH National Center for Research Resources (NCRR)
- Language
- English
- Date published
- 11/03/2015
- Academic Unit
- Microbiology and Immunology
- Record Identifier
- 9984827337002771
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