Structural Characterization of a Flavonoid-Inducible Pseudomonas aeruginosa A-Band-Like O Antigen of Rhizobium sp. Strain NGR234, Required for the Formation of Nitrogen-Fixing Nodules

Bradley L Reuhs; Biserka Relić; L. Scott Forsberg; Corinne Marie; Tuula Ojanen-Reuhs; Samuel B Stephens; Chee-Hoong Wong; Saïd Jabbouri; William J Broughton

doi:10.1128/JB.187.18.6479-6487.2005

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Structural Characterization of a Flavonoid-Inducible Pseudomonas aeruginosa A-Band-Like O Antigen of Rhizobium sp. Strain NGR234, Required for the Formation of Nitrogen-Fixing Nodules

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Structural Characterization of a Flavonoid-Inducible Pseudomonas aeruginosa A-Band-Like O Antigen of Rhizobium sp. Strain NGR234, Required for the Formation of Nitrogen-Fixing Nodules

Bradley L Reuhs, Biserka Relić, L. Scott Forsberg, Corinne Marie, Tuula Ojanen-Reuhs, Samuel B Stephens, Chee-Hoong Wong, Saïd Jabbouri and William J Broughton

Journal of bacteriology, Vol.187(18), pp.6479-6487

09/2005

DOI: 10.1128/JB.187.18.6479-6487.2005

PMCID: PMC1236632

PMID: 16159781

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Abstract

Rhizobium ( Sinorhizobium ) sp. strain NGR234 contains three replicons, the smallest of which (pNGR234 a ) carries most symbiotic genes, including those required for nodulation and lipo-chito-oligosaccharide (Nod factor) biosynthesis. Activation of nod gene expression depends on plant-derived flavonoids, NodD transcriptional activators, and nod box promoter elements. Nod boxes NB6 and NB7 delimit six different types of genes, one of which ( fixF ) is essential for the formation of effective nodules on Vigna unguiculata . In vegetative culture, wild-type NGR234 produces a distinct, flavonoid-inducible lipopolysaccharide (LPS) that is not produced by the mutant (NGRΩ fixF ); this LPS is also found in nitrogen-fixing bacteroids isolated from V. unguiculata infected with NGR234. Electron microscopy showed that peribacteroid membrane formation is perturbed in nodule cells infected by the fixF mutant. LPSs were purified from free-living NGR234 cultured in the presence of apigenin. Structural analyses showed that the polysaccharide portions of these LPSs are specialized, rhamnose-containing O antigens attached to a modified core-lipid A carrier. The primary sequence of the O antigen is [-3)-α- l -Rha p -(1,3)-α- l -Rha p -(1,2)-α- l -Rha p -(1-] n , and the LPS core region lacks the acidic sugars commonly associated with the antigenic outer core of LPS from noninduced cells. This rhamnan O antigen, which is absent from noninduced cells, has the same primary sequence as the A-band O antigen of Pseudomonas aeruginosa , except that it is composed of l -rhamnose rather than the d -rhamnose characteristic of the latter. It is noteworthy that A-band LPS is selectively maintained on the P. aeruginosa cell surface during chronic cystic fibrosis lung infection, where it is associated with an increased duration of infection.

Plant Microbiology

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Title: Subtitle: Structural Characterization of a Flavonoid-Inducible Pseudomonas aeruginosa A-Band-Like O Antigen of Rhizobium sp. Strain NGR234, Required for the Formation of Nitrogen-Fixing Nodules
Creators: Bradley L Reuhs - Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, 1160 Food Science Building, West Lafayette, Indiana 47907-1160
Biserka Relić - Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, 1160 Food Science Building, West Lafayette, Indiana 47907-1160
L. Scott Forsberg - Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, 1160 Food Science Building, West Lafayette, Indiana 47907-1160
Corinne Marie - Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, 1160 Food Science Building, West Lafayette, Indiana 47907-1160
Tuula Ojanen-Reuhs - Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, 1160 Food Science Building, West Lafayette, Indiana 47907-1160
Samuel B Stephens - Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, 1160 Food Science Building, West Lafayette, Indiana 47907-1160
Chee-Hoong Wong - Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, 1160 Food Science Building, West Lafayette, Indiana 47907-1160
Saïd Jabbouri - Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, 1160 Food Science Building, West Lafayette, Indiana 47907-1160
William J Broughton - Whistler Center for Carbohydrate Research, Department of Food Science, Purdue University, 1160 Food Science Building, West Lafayette, Indiana 47907-1160
Resource Type: Journal article
Publication Details: Journal of bacteriology, Vol.187(18), pp.6479-6487
DOI: 10.1128/JB.187.18.6479-6487.2005
PMID: 16159781
PMCID: PMC1236632
NLM abbreviation: J Bacteriol
ISSN: 0021-9193
eISSN: 1098-5530
Publisher: American Society for Microbiology
Language: English
Date published: 09/2005
Academic Unit: Fraternal Order of Eagles Diabetes Research Center; Endocrinology and Metabolism; Internal Medicine
Record Identifier: 9984094334302771

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