Journal article
Structural Characterization of a Soluble Amyloid beta-Peptide Oligomer
Biochemistry (Easton), Vol.48(9), pp.1870-1877
03/10/2009
DOI: 10.1021/bi802046n
PMID: 19216516
Abstract
Alzheimer's disease (AD) is a neurodegenerative disorder that is linked to the presence of amyloid beta-peptides that can form insoluble fibrils or soluble oligomeric assemblies. Soluble forms are present in the brains and tissues of Alzheimer's patients, and their presence correlates with disease progression. Long-lived soluble forms can be generated in vitro by using small amounts of aliphatic hydrocarbon chains of detergents or fatty acids in preparations of amyloid beta-peptides. Using NMR, we have characterized soluble oligomers of A beta preglobulomer and globulomer that are stable and alter synaptic activity. The NMR data indicate that these soluble forms have a mixed parallel and antiparallel beta-sheet structure that is different from fibrils which contain only parallel beta-sheets. Using the structural data, we engineered a disulfide bond into the soluble A beta globulomer to give a "new" soluble antigen that is stable, homogeneous, and binds with the same affinity to selective antibodies as the parent wt globulomer.
Details
- Title: Subtitle
- Structural Characterization of a Soluble Amyloid beta-Peptide Oligomer
- Creators
- Liping Yu - AbbottRohinton Edalji - AbbottJohn E. Harlan - AbbottThomas F. Holzman - AbbottAna Pereda Lopez - AbbottBoris Labkovsky - AbbottHeinz Hillen - AbbottStefan Barghorn - AbbottUlrich Ebert - AbbottPaul L. Richardson - AbbottLaura Miesbauer - AbbottLarry Solomon - AbbottDiane Bartley - AbbottKarl Walter - AbbottRobert W. Johnson - AbbottPhilip J. Hajduk - AbbottEdward T. Olejniczak - Abbott
- Resource Type
- Journal article
- Publication Details
- Biochemistry (Easton), Vol.48(9), pp.1870-1877
- Publisher
- Amer Chemical Soc
- DOI
- 10.1021/bi802046n
- PMID
- 19216516
- ISSN
- 0006-2960
- eISSN
- 1520-4995
- Number of pages
- 8
- Language
- English
- Date published
- 03/10/2009
- Academic Unit
- Biochemistry and Molecular Biology; Medicine Administration
- Record Identifier
- 9984627350402771
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