Journal article
Structural and Biomechanical Changes to Dentin Extracellular Matrix Following Chemical Removal of Proteoglycans
Odontology, Vol.107(3), pp.316-323
02/01/2019
DOI: 10.1007/s10266-018-00408-0
PMCID: PMC6559829
PMID: 30710179
Abstract
Proteoglycans are biomacromolecules with significant biomineralization and structural roles in the dentin extracellular matrix. This study comprehensively assessed the mechanical properties and morphology of the dentin extracellular matrix following chemical removal of proteoglycans to elucidate the structural roles of proteoglycans in dentin. Dentin extracellular matrix was prepared from extracted teeth after complete tissue demineralization. Chemical removal of proteoglycans was carried-out using guanidine hydrochloride (GuHCl) for up to 10 days. The removal of proteoglycans was determined by dimethylmethylene blue colorimetric assay and histological staining analyses using transmission electron microscopy and optical microscopy. The modulus of elasticity of dentin matrix was determined by a 3-point bending test method. Partial removal of proteoglycans induced significant modifications to the dentin matrix, particularly in the type I collagen. Removal of proteoglycans significantly decreased the modulus of elasticity of dentin extracellular matrix (p < 0.0001). In conclusion, the subtle disruption of proteoglycans induce pronounced changes to the collagen network packing and the bulk modulus of elasticity of dentin matrix.
Details
- Title: Subtitle
- Structural and Biomechanical Changes to Dentin Extracellular Matrix Following Chemical Removal of Proteoglycans
- Creators
- Ana Paula Farina - University of Illinois ChicagoCristina M. P. Vidal - University of IowaDoglas Cecchin - University of Illinois ChicagoThaiane R. Aguiar - Universidade Federal da BahiaAna Karina Bedran-Russo - University of Illinois Chicago
- Resource Type
- Journal article
- Publication Details
- Odontology, Vol.107(3), pp.316-323
- DOI
- 10.1007/s10266-018-00408-0
- PMID
- 30710179
- PMCID
- PMC6559829
- NLM abbreviation
- Odontology
- ISSN
- 1618-1247
- eISSN
- 1618-1255
- Grant note
- DOI: 10.13039/100000072, name: National Institute of Dental and Craniofacial Research, award: DE21040; name: Coordena??o de Aperfei?oamento de Pessoal de N?vel Superior, award: BEX 17764/12-2
- Language
- English
- Date published
- 02/01/2019
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984367736602771
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