Journal article
Structural and Functional Microvascular Alterations in a Rat Model of Metabolic Syndrome Induced by a High-Fat Diet
Obesity (Silver Spring, Md.), Vol.21(10), pp.2046-2054
10/2013
DOI: 10.1002/oby.20358
PMID: 23512529
Abstract
Objective: To investigate microvascular alterations in an experimental model of metabolic syndrome induced by a high-fat diet (HFD) associated with salt supplementation (0.5% NaCl).
Design and Methods: Wistar Kyoto rats were fed standard chow (control group, CONT) or HFD for 20 weeks. The functional capillary density (FCD) was assessed using intravital fluorescence videomicroscopy.
Results: The HFD group presented a higher systolic blood pressure, plasma glucose and insulin levels, total and LDL-cholesterol levels, triglycerides, and visceral and epididymal fat when compared with the CONT group. When compared with the CONT group, the HFD group showed a lower FCD in the skeletal muscle (P < 0.05) but not in the skin (P > 0.05). The HFD group also had a lower capillary-to-fiber ratio in the skeletal muscle (P < 0.01). The capillary volume density-to-fiber volume density ratio in the left ventricle of the HFD was also reduced (P < 0.01). Finally, rats fed with HFD showed ventricular hypertrophy and increased cardiomyocyte diameter (P < 0.01).
Conclusions: The long-term administration of a HFD associated with salt supplementation to rats generates an experimental model of metabolic syndrome characterized by central body fat deposition, insulin resistance, glucose intolerance, hypertriglyceridemia, hypercholesterolemia, arterial hypertension, cardiac remodeling, and rarefaction of the microcirculation in the heart and skeletal muscle.
Details
- Title: Subtitle
- Structural and Functional Microvascular Alterations in a Rat Model of Metabolic Syndrome Induced by a High-Fat Diet
- Creators
- Alessandro R. Nascimento - Fundação Oswaldo CruzMarcus Machado - Fiocruz MS, Inst Oswaldo Cruz, Lab Cardiovasc Invest, BR-21045900 Rio De Janeiro, BrazilNathalia de Jesus - Fundação Oswaldo CruzFabiana Gomes - Fiocruz MS, Inst Oswaldo Cruz, Lab Cardiovasc Invest, BR-21045900 Rio De Janeiro, BrazilMarcos A. Lessa - Fundação Oswaldo CruzIsabela T. Bonomo - Fundação Oswaldo CruzEduardo Tibirica - Fundação Oswaldo Cruz
- Resource Type
- Journal article
- Publication Details
- Obesity (Silver Spring, Md.), Vol.21(10), pp.2046-2054
- DOI
- 10.1002/oby.20358
- PMID
- 23512529
- NLM abbreviation
- Obesity (Silver Spring)
- ISSN
- 1930-7381
- eISSN
- 1930-739X
- Publisher
- Wiley
- Number of pages
- 9
- Language
- English
- Date published
- 10/2013
- Academic Unit
- Anesthesia
- Record Identifier
- 9984656610302771
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