Journal article
Structural basis of gap-filling DNA synthesis in the nucleosome by DNA Polymerase β
Nature communications, Vol.16(1), 2607
03/17/2025
DOI: 10.1038/s41467-025-57915-2
PMCID: PMC11914125
PMID: 40097433
Abstract
Single-strand breaks (SSBs) are one of the most prevalent forms of DNA damage found in the chromatinized genome and are repaired by single-strand break repair (SSBR) or base excision repair (BER). DNA polymerase beta (Pol β) is the primary enzyme responsible for processing the 1-nt gap intermediate in chromatin during SSBR and BER. To date, the mechanism used by Pol β to process a 1-nt gap in the context of chromatin remains poorly understood. Here, we use biochemical assays and cryogenic electron microscopy (cryo-EM) to determine the kinetic and structural basis of gap-filling DNA synthesis in the nucleosome by Pol β. This work establishes that Pol β uses a global DNA sculpting mechanism for processing 1-nt gaps in the nucleosome during SSBR and BER, providing fundamental insight into DNA repair in chromatin.
DNA polymerase beta (Pol β) is the primary enzyme responsible for processing 1-nt gaps in chromatin during DNA repair. Here, the authors determine the kinetic and structural basis of gap-filling DNA synthesis in the nucleosome by Pol β, providing fundamental insight into DNA repair in chromatin.
Details
- Title: Subtitle
- Structural basis of gap-filling DNA synthesis in the nucleosome by DNA Polymerase β
- Creators
- Tyler M. Weaver - University of Kansas Medical CenterBenjamin J. Ryan - University of Kansas Medical CenterSpencer H. Thompson - University of Kansas Medical CenterAdil S. Hussen - University of Kansas Medical CenterJonah J. Spencer - University of Kansas Medical CenterZhen Xu - University of IowaNicholas J. Schnicker - University of IowaBret D. Freudenthal - The University of Kansas Cancer Center
- Resource Type
- Journal article
- Publication Details
- Nature communications, Vol.16(1), 2607
- DOI
- 10.1038/s41467-025-57915-2
- PMID
- 40097433
- PMCID
- PMC11914125
- NLM abbreviation
- Nat Commun
- ISSN
- 2041-1723
- eISSN
- 2041-1723
- Publisher
- Nature Publishing Group UK
- Grant note
- R35GM128562 / U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) (https://doi.org/10.13039/100000057)
- Language
- English
- Date published
- 03/17/2025
- Academic Unit
- Molecular Physiology and Biophysics; Medicine Administration
- Record Identifier
- 9984801838102771
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