Structural transition of kidney cystatin induced by silicon dioxide nanoparticles: An implication for renal diseases

Anas Shamsi; Azaj Ahmed; Bilqees Bano

doi:10.1016/j.ijbiomac.2016.10.019

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Structural transition of kidney cystatin induced by silicon dioxide nanoparticles: An implication for renal diseases

Journal article

Peer reviewed

Structural transition of kidney cystatin induced by silicon dioxide nanoparticles: An implication for renal diseases

Anas Shamsi, Azaj Ahmed and Bilqees Bano

International journal of biological macromolecules, Vol.94(Pt B), pp.754-761

01/2017

DOI: 10.1016/j.ijbiomac.2016.10.019

PMID: 27771415

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Abstract

Nanotechnology is one of the fastest growing fields of science owing to use of nanomaterials in industries and medicine across the globe. Currently silicon dioxide nanoparticles (SiO NPs) are one of the most popular nanomaterials owing to their inert toxicity profile and hence exposure to SiO nanoparticles is on the increase. Cystatins are thiol proteinase inhibitors (TPIs) ubiquitously distributed in plants and animals and they are now at the heed of a number of normal and pathological conditions and shouldn't be regarded solely as TPIs. Up till now many studies have targeted the potential toxicity of NPs on pulmonary target; although little focus is given to kidney which is a secondary target organ. The objective of this work is to study the structural changes in buffalo kidney cystatin (BKC) induced by SiO NPs. UV and Fluorescence spectroscopy shows BKC transformation from native to non-native form evident by decreased absorbance and increased fluorescence. FTIR and CD spectroscopy further confirmed secondary structure disruption of BKC. Isothermal titration calorimetry (ITC) and microscopy were resorted to visualize interaction between SiO NPs and BKC. Comet assay and MTT assay were utilized to perceive the toxicity of SiO NPs incubated BKC; decreased cell viability clearly suggesting toxicity of SiO NPs incubated BKC. Our work suggests that SiO NPs have a deteriorating effect on BKC thereby causing a decrease in its ability to inhibit papain and hence less functionality. This study also shows that BKC transforms to a toxic non-native form in presence of SiO NPs.

Animals

Buffaloes

Cell Survival - drug effects

Comet Assay

Cystatins - chemistry

Cystatins - isolation & purification

Cystatins - pharmacology

Cysteine Proteinase Inhibitors - chemistry

Cysteine Proteinase Inhibitors - isolation & purification

Cysteine Proteinase Inhibitors - pharmacology

Humans

Kidney - chemistry

Lymphocytes - cytology

Lymphocytes - drug effects

Nanoparticles - chemistry

Nanoparticles - toxicity

Nanoparticles - ultrastructure

Nucleic Acid Denaturation - drug effects

Primary Cell Culture

Protein Binding

Protein Structure, Secondary

Silicon Dioxide - chemistry

Details

Title: Subtitle: Structural transition of kidney cystatin induced by silicon dioxide nanoparticles: An implication for renal diseases
Creators: Anas Shamsi - Aligarh Muslim University
Azaj Ahmed - Aligarh Muslim University
Bilqees Bano - Aligarh Muslim University
Resource Type: Journal article
Publication Details: International journal of biological macromolecules, Vol.94(Pt B), pp.754-761
DOI: 10.1016/j.ijbiomac.2016.10.019
PMID: 27771415
ISSN: 0141-8130
eISSN: 1879-0003
Language: English
Date published: 01/2017
Academic Unit: Internal Medicine
Record Identifier: 9985177941102771

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