Journal article
Structure-activity Relationships for Hydroxylated Polychlorinated Biphenyls as Inhibitors of the Sulfation of Dehydroepiandrosterone Catalyzed by Human Hydroxysteroid Sulfotransferase SULT2A1
Chemical research in toxicology, Vol.24(10), pp.1720-1728
10/17/2011
DOI: 10.1021/tx200260h
PMCID: PMC3196794
PMID: 21913674
Abstract
Polychlorinated biphenyls (PCBs) are persistent worldwide pollutants that are of concern due to their bioaccumulation and health effects. Metabolic oxidation of PCBs results in the formation of hydroxylated metabolites (OHPCBs). Among their biological effects, OHPCBs have been shown to alter the metabolism of endocrine hormones, including inhibition of mammalian cytosolic sulfotransferases (SULTs) that are responsible for the inactivation of thyroid hormones and phenolic steroids (i.e., hSULT1A1, hSULT1B1, and hSULT1E1). OHPCBs also interact with a human hydroxysteroid sulfotransferase that plays a role in the sulfation of endogenous alcohol-containing steroid hormones and bile acids (i.e., hSULT2A1). The objectives of our current study were to examine the effects of a series of OHPCB congeners on the activity of hSULT2A1 and to develop a three-dimensional quantitative structure activity relationship (3D-QSAR) model for OHPCBs as inhibitors of the enzyme. A total of 15 OHPCBs were examined, and the sulfation of 1 μM [
3
H] dehydroepiandrosterone (DHEA) was utilized as a model reaction catalyzed by the enzyme. All 15 OHPCBs inhibited the sulfation of DHEA, with IC
50
values ranging from 0.6 μM to 96 μM, and eight of these OHPCBs were also substrates for the enzyme. Comparative molecular field analysis (CoMFA) provided a predictive 3D-QSAR model with a q
2
value of 0.697 and an r
2
value of 0.949. The OHPCBs that had the highest potency as inhibitors of DHEA sulfation were those with a 3, 5-dichloro-4-hydroxy substitution pattern on the biphenyl ring system, and these congeners were also substrates for sulfation catalyzed by hSULT2A1.
Details
- Title: Subtitle
- Structure-activity Relationships for Hydroxylated Polychlorinated Biphenyls as Inhibitors of the Sulfation of Dehydroepiandrosterone Catalyzed by Human Hydroxysteroid Sulfotransferase SULT2A1
- Creators
- Edugie J Ekuase - Department of Occupational and Environmental Health, College of Public Health, The University of Iowa, Iowa City, Iowa 52242Yungang Liu - Department of Occupational and Environmental Health, College of Public Health, The University of Iowa, Iowa City, Iowa 52242Hans-Joachim Lehmler - Department of Occupational and Environmental Health, College of Public Health, The University of Iowa, Iowa City, Iowa 52242Larry W Robertson - Department of Occupational and Environmental Health, College of Public Health, The University of Iowa, Iowa City, Iowa 52242Michael W Duffel - Department of Occupational and Environmental Health, College of Public Health, The University of Iowa, Iowa City, Iowa 52242
- Resource Type
- Journal article
- Publication Details
- Chemical research in toxicology, Vol.24(10), pp.1720-1728
- DOI
- 10.1021/tx200260h
- PMID
- 21913674
- PMCID
- PMC3196794
- NLM abbreviation
- Chem Res Toxicol
- ISSN
- 0893-228X
- eISSN
- 1520-5010
- Grant note
- R01 CA038683-25 || CA / National Cancer Institute : NCI P30 ES005605-21 || ES / National Institute of Environmental Health Sciences : NIEHS P42 ES013661-05 || ES / National Institute of Environmental Health Sciences : NIEHS
- Language
- English
- Date published
- 10/17/2011
- Academic Unit
- Occupational and Environmental Health; Iowa Neuroscience Institute; Pharmaceutical Sciences and Experimental Therapeutics; Iowa Superfund Research Program; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984001096502771
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