Journal article
Structure and mapping of the human β-defensin HBD-2 gene and its expression at sites of inflammation
Gene, Vol.222(2), pp.237-244
1998
DOI: 10.1016/S0378-1119(98)00480-6
PMID: 9831658
Abstract
We cloned a second human β-defensin gene, HBD-2, and determined its gene structure and expression in inflamed tissue sections. The entire gene spanned about 2
kb with two small exons and one intron. Radiation hybrid studies confirmed the location on chromosome 8p, were consistent with the order HNP-1, HBD-1 and HBD-2, and located HBD-2 as the most centromeric of the genes. By three-color fluorescence in situ hybridization on both free chromatin fiber mapping and interphase mapping, HBD-1, HBD-2 and HNP-1 were mapped to chromosome 8p23. HBD-1 was within 40–100
kb of HNP-1, while HBD-2 was about 500–600
kb from HBD-1, with the most likely order HNP-1, HBD-1, HBD-2. The expression of HBD-2 was locally regulated by inflammation. HBD-2 mRNA was markedly increased in the epidermis surrounding inflamed regions, but not detectable in adjacent non-inflamed areas, a distribution that was confirmed at the peptide level by immunostaining with HBD-2 antibody. The HBD-2 gene is the first member of the human defensin family that is locally inducible by inflammation.
Details
- Title: Subtitle
- Structure and mapping of the human β-defensin HBD-2 gene and its expression at sites of inflammation
- Creators
- Paul B McCray - Department of Pediatrics, University of Iowa, Iowa City, IA, USALide Liu - Departments of Medicine and Pathology and the Will Rogers Institute for Pulmonary Research, UCLA School of Medicine, Los Angeles, CA, USALina Wang - Department of Pathology, Harbor-UCLA Medical Center, Los Angeles, CA, USAHong Peng Jia - Department of Pediatrics, University of Iowa, Iowa City, IA, USAChengquan Zhao - Departments of Medicine and Pathology and the Will Rogers Institute for Pulmonary Research, UCLA School of Medicine, Los Angeles, CA, USAHenry H.Q Heng - SeeDNABiotech, Inc. and Department of Biology, York University, North York, Ont. M3J 1P3, CanadaBrian C Schutte - Department of Pediatrics, University of Iowa, Iowa City, IA, USATomas Ganz - Departments of Medicine and Pathology and the Will Rogers Institute for Pulmonary Research, UCLA School of Medicine, Los Angeles, CA, USA
- Resource Type
- Journal article
- Publication Details
- Gene, Vol.222(2), pp.237-244
- Publisher
- Elsevier B.V
- DOI
- 10.1016/S0378-1119(98)00480-6
- PMID
- 9831658
- ISSN
- 0378-1119
- eISSN
- 1879-0038
- Language
- English
- Date published
- 1998
- Academic Unit
- Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Internal Medicine
- Record Identifier
- 9984093334002771
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