Structure of the Golgi apparatus is not influenced by a GAG deletion mutation in the dystonia-associated gene Tor1a

Sara B Mitchell; Sadahiro Iwabuchi; Hiroyuki Kawano; Tsun Ming Tom Yuen; Jin-Young Koh; K. W. David Ho; N. Charles Harata

doi:10.1371/journal.pone.0206123

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Structure of the Golgi apparatus is not influenced by a GAG deletion mutation in the dystonia-associated gene Tor1a

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Structure of the Golgi apparatus is not influenced by a GAG deletion mutation in the dystonia-associated gene Tor1a

Sara B Mitchell, Sadahiro Iwabuchi, Hiroyuki Kawano, Tsun Ming Tom Yuen, Jin-Young Koh, K. W. David Ho and N. Charles Harata

PloS one, Vol.13(11), pp.e0206123-e0206123

11/01/2018

DOI: 10.1371/journal.pone.0206123

PMCID: PMC6221310

PMID: 30403723

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Abstract

Autosomal-dominant, early-onset DYT1 dystonia is associated with an in-frame deletion of a glutamic acid codon (ΔE) in the TOR1A gene. The gene product, torsinA, is an evolutionarily conserved AAA+ ATPase. The fact that constitutive secretion from patient fibroblasts is suppressed indicates that the ΔE-torsinA protein influences the cellular secretory machinery. However, which component is affected remains unclear. Prompted by recent reports that abnormal protein trafficking through the Golgi apparatus, the major protein-sorting center of the secretory pathway, is sometimes associated with a morphological change in the Golgi, we evaluated the influence of ΔE-torsinA on this organelle. Specifically, we examined its structure by confocal microscopy, in cultures of striatal, cerebral cortical and hippocampal neurons obtained from wild-type, heterozygous and homozygous ΔE-torsinA knock-in mice. In live neurons, the Golgi was assessed following uptake of a fluorescent ceramide analog, and in fixed neurons it was analyzed by immuno-fluorescence staining for the Golgi-marker GM130. Neither staining method indicated genotype-specific differences in the size, staining intensity, shape or localization of the Golgi. Moreover, no genotype-specific difference was observed as the neurons matured in vitro . These results were supported by a lack of genotype-specific differences in GM130 expression levels, as assessed by Western blotting. The Golgi was also disrupted by treatment with brefeldin A, but no genotype-specific differences were found in the immuno-fluorescence staining intensity of GM130. Overall, our results demonstrate that the ΔE-torsinA protein does not drastically influence Golgi morphology in neurons, irrespective of genotype, brain region (among those tested), or maturation stage in culture. While it remains possible that functional changes in the Golgi exist, our findings imply that any such changes are not severe enough to influence its morphology to a degree detectable by light microscopy.

Medicine and Health Sciences

Biology and Life Sciences

Research and Analysis Methods

Details

Title: Subtitle: Structure of the Golgi apparatus is not influenced by a GAG deletion mutation in the dystonia-associated gene Tor1a
Creators: Sara B Mitchell
Sadahiro Iwabuchi
Hiroyuki Kawano
Tsun Ming Tom Yuen
Jin-Young Koh
K. W. David Ho
N. Charles Harata
Resource Type: Journal article
Publication Details: PloS one, Vol.13(11), pp.e0206123-e0206123
DOI: 10.1371/journal.pone.0206123
PMID: 30403723
PMCID: PMC6221310
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science; San Francisco, CA USA
Grant note: ; W81XWH-14-1-0301 / ;
Alternative title: TorsinA and Golgi apparatus structure
Language: English
Date published: 11/01/2018
Academic Unit: Molecular Physiology and Biophysics; Biochemistry and Molecular Biology; Otolaryngology
Record Identifier: 9984071695302771

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