Journal article
Structure of the N-Terminal RNA-Binding Domain of the SARS CoV Nucleocapsid Protein
Biochemistry (Easton), Vol.43(20), pp.6059-6063
05/25/2004
DOI: 10.1021/bi036155b
PMID: 15147189
Abstract
The severe acute respiratory syndrome (SARS) virus belongs to the Coronaviridea family of viruses. Its virion encodes several proteins including a replicase and four structural proteins. Here we describe the three-dimensional structure of the N-terminal domain of the SARS coronavirus (CoV) nucleocapsid protein. The protein consists of a five-stranded β sheet with a folding topology distinct from other RNA-binding proteins. Single-stranded RNAs bind to the protein surface at the junction between a flexible, positively charged β hairpin and the core structure. NMR-based screening was used to identify low molecular weight compounds that bind to this site.
Details
- Title: Subtitle
- Structure of the N-Terminal RNA-Binding Domain of the SARS CoV Nucleocapsid Protein
- Creators
- Qiulong Huang - Abbott (United States)Liping Yu - Abbott (United States)Andrew M. Petros - Abbott (United States)Angelo Gunasekera - Abbott (United States)Zhihong Liu - Abbott (United States)Nan Xu - Abbott (United States)Philip Hajduk - Abbott (Germany)Jamey Mack - Abbott (United States)Stephen W. Fesik - Abbott (United States)Edward T. Olejniczak - Abbott (Germany)
- Resource Type
- Journal article
- Publication Details
- Biochemistry (Easton), Vol.43(20), pp.6059-6063
- DOI
- 10.1021/bi036155b
- PMID
- 15147189
- NLM abbreviation
- Biochemistry
- ISSN
- 0006-2960
- eISSN
- 1520-4995
- Language
- English
- Date published
- 05/25/2004
- Academic Unit
- Biochemistry and Molecular Biology; Medicine Administration
- Record Identifier
- 9984627328702771
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