Journal article
Structure of the N-terminal region of Haemophilus Influenzae HI0017: Implications for function
Journal of biomolecular NMR, Vol.20(2), pp.105-110
06/01/2001
DOI: 10.1023/A:1011264300726
PMID: 11495242
Abstract
Haemophilus influenzae is a gram-negative pathogen that causes infections ranging from asymptomatic colonization of the human upper respiratory tract to serious invasive diseases such as meningitis. Although the genome of Haemophilus influenzae has been completely sequenced, the structure and function of many of these proteins are unknown. HI0017 is one of these uncharacterized proteins. Here we describe the three-dimensional solution structure of the N-terminal portion of HI0017 as determined by NMR spectroscopy. The structure consists of a five-stranded antiparallel β-sheet and two short α-helices. It is similar to the C-terminal domain of Diphtheria toxin repressor (DtxR). The C-terminal portion of HI0017 has an amino acid sequence that closely resembles pyruvate formate-lyase - an enzyme that converts pyruvate and CoA into acetyl-CoA and formate by a radical mechanism. Based on structural and sequence comparisons, we propose that the C-terminus of HI0017 functions as an enzyme with a glycyl radical mechanism, while the N-terminus participates in protein/protein interactions involving an activase (iron-sulfur protein) and/or the substrate.[PUBLICATION ABSTRACT]
Details
- Title: Subtitle
- Structure of the N-terminal region of Haemophilus Influenzae HI0017: Implications for function
- Creators
- Liping Yu - Discovery LaboratoriesJamey Mack - AbbottPhil Hajduk - Discovery LaboratoriesStephen Fesik - Discovery Laboratories
- Resource Type
- Journal article
- Publication Details
- Journal of biomolecular NMR, Vol.20(2), pp.105-110
- Publisher
- Springer Nature B.V
- DOI
- 10.1023/A:1011264300726
- PMID
- 11495242
- ISSN
- 0925-2738
- eISSN
- 1573-5001
- Language
- English
- Date published
- 06/01/2001
- Academic Unit
- Biochemistry and Molecular Biology; Medicine Administration
- Record Identifier
- 9984627215802771
Metrics
4 Record Views