Journal article
Structure of the Protein Phosphatase 2A Holoenzyme
Cell (Cambridge), Vol.127(6), pp.1239-1251
2006
DOI: 10.1016/j.cell.2006.11.033
PMID: 17174897
Abstract
Protein Phosphatase 2A (PP2A) plays an essential role in many aspects of cellular physiology. The PP2A holoenzyme consists of a heterodimeric core enzyme, which comprises a scaffolding subunit and a catalytic subunit, and a variable regulatory subunit. Here we report the crystal structure of the heterotrimeric PP2A holoenzyme involving the regulatory subunit B′/B56/PR61. Surprisingly, the B′/PR61 subunit has a HEAT-like (
huntingtin-
elongation-
A subunit-
TOR-like) repeat structure, similar to that of the scaffolding subunit. The regulatory B′/B56/PR61 subunit simultaneously interacts with the catalytic subunit as well as the conserved ridge of the scaffolding subunit. The carboxyterminus of the catalytic subunit recognizes a surface groove at the interface between the B′/B56/PR61 subunit and the scaffolding subunit. Compared to the scaffolding subunit in the PP2A core enzyme, formation of the holoenzyme forces the scaffolding subunit to undergo pronounced conformational rearrangements. This structure reveals significant ramifications for understanding the function and regulation of PP2A.
Details
- Title: Subtitle
- Structure of the Protein Phosphatase 2A Holoenzyme
- Creators
- Yanhui Xu - Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, Princeton, NJ 08544, USAYongna Xing - Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, Princeton, NJ 08544, USAYu Chen - Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, Princeton, NJ 08544, USAYang Chao - Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, Princeton, NJ 08544, USAZheng Lin - Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, Princeton, NJ 08544, USAEugene Fan - Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, Princeton, NJ 08544, USAJong W Yu - Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, Princeton, NJ 08544, USAStefan Strack - Department of Pharmacology, University of Iowa Carver College of Medicine, Iowa City, IA 52242, USAPhilip D Jeffrey - Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, Princeton, NJ 08544, USAYigong Shi - Department of Molecular Biology, Lewis Thomas Laboratory, Princeton University, Princeton, NJ 08544, USA
- Resource Type
- Journal article
- Publication Details
- Cell (Cambridge), Vol.127(6), pp.1239-1251
- Publisher
- Elsevier Inc
- DOI
- 10.1016/j.cell.2006.11.033
- PMID
- 17174897
- ISSN
- 0092-8674
- eISSN
- 1097-4172
- Language
- English
- Date published
- 2006
- Academic Unit
- Pathology; Iowa Neuroscience Institute; Neuroscience and Pharmacology
- Record Identifier
- 9984040499202771
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