Structure of the murine Jak2 protein-tyrosine kinase and its role in interleukin 3 signal transduction

Olli Silvennoinen; Bruce A Witthuhn; Frederick W Quelle; John L Cleveland; Taolin Yi; James N Ihle

doi:10.1073/pnas.90.18.8429

Back

Structure of the murine Jak2 protein-tyrosine kinase and its role in interleukin 3 signal transduction

Journal article

Open access

Peer reviewed

Structure of the murine Jak2 protein-tyrosine kinase and its role in interleukin 3 signal transduction

Olli Silvennoinen, Bruce A Witthuhn, Frederick W Quelle, John L Cleveland, Taolin Yi and James N Ihle

Proceedings of the National Academy of Sciences - PNAS, Vol.90(18), pp.8429-8433

09/15/1993

DOI: 10.1073/pnas.90.18.8429

PMCID: PMC47370

PMID: 8378315

Files and links (1)

url

https://doi.org/10.1073/pnas.90.18.8429View

Published (Version of record) Open Access

Abstract

Interleukin 3 (IL-3) regulates the proliferation and differentiation of hematopoietic cells. Although the IL-3 receptor chains lack kinase catalytic domains, IL-3 induces tyrosine phosphorylation of cellular proteins. To investigate the potential role of the JAK family of protein-tyrosine kinases in IL-3 signal transduction, we have obtained full-length cDNA clones for murine Jak1 and Jak2 protein-tyrosine kinases and prepared antiserum against the predicted proteins. Using antisera against Jak2, we demonstrate that IL-3 stimulation results in the rapid and specific tyrosine phosphorylation of Jak2 and activates its in vitro kinase activity.

Details

Title: Subtitle: Structure of the murine Jak2 protein-tyrosine kinase and its role in interleukin 3 signal transduction
Creators: Olli Silvennoinen - Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, TN 38105
Bruce A Witthuhn - Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, TN 38105
Frederick W Quelle - Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, TN 38105
John L Cleveland - Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, TN 38105
Taolin Yi - Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, TN 38105
James N Ihle - Department of Biochemistry, St. Jude Children's Research Hospital, Memphis, TN 38105
Resource Type: Journal article
Publication Details: Proceedings of the National Academy of Sciences - PNAS, Vol.90(18), pp.8429-8433
DOI: 10.1073/pnas.90.18.8429
PMID: 8378315
PMCID: PMC47370
NLM abbreviation: Proc Natl Acad Sci U S A
ISSN: 0027-8424
eISSN: 1091-6490
Publisher: National Academy of Sciences
Language: English
Date published: 09/15/1993
Academic Unit: Neuroscience and Pharmacology; Internal Medicine
Record Identifier: 9984040495102771

Metrics

20 Record Views

540 Times Cited - Web of Science

See more details