Journal article
Structure–function relationships of insulin-like growth factor binding protein 6 (IGFBP-6) and its chimeras
Growth hormone & IGF research, Vol.12(2), pp.91-98
04/01/2002
DOI: 10.1054/ghir.2001.0266
PMID: 12175646
Abstract
Insulin-like growth factor binding protein 6 (IGFBP-6) is a high-affinity IGFBP with substantially greater affinity for insulin-like growth factor-II (IGF-II) than IGF-I. IGFBP-63 is a chimera which has a 20 amino acidC -terminal portion of IGFBP-6 switched with the homologous area of IGFBP-3, P3. Unlike IGFBP-43, in which the P3 region was exchanged for the homologous region of IGFBP-4 (P4), IGFBP-63 does not bind to endothelial cells. Double mutations were made with the P3 region exchanged as well as a second area differing from IGFBP-3 to form IGFBP-63A and IGFBP-63B, by replacing this area with the homologous region of IGFBP-3. Neither [125I]IGFBP-63A nor IGFBP-63B specifically bound to endothelial cells. However, each double mutant competed for [125I]IGFBP-3 binding to cultured cells. In the perfused heart, transendothelial transport of IGFBP-6 and IGFBP-63 was only 25% of similar transendothelial transport of perfused IGFBP-3. We conclude that chimeras of IGFBP-6 and IGFBP-36 clearly differ from IGFBP-43 in their ability to bind specifically to endothelial cells and in their capacity to undergo transendothelial transportation in the perfused heart.
Details
- Title: Subtitle
- Structure–function relationships of insulin-like growth factor binding protein 6 (IGFBP-6) and its chimeras
- Creators
- M Boes - Iowa Diabetes and Endocrinology Research CenterB.L Dake - University of IowaB.A Booth - Iowa Diabetes and Endocrinology Research CenterA Sandra - Iowa Diabetes and Endocrinology Research CenterM Bateman - Iowa Diabetes and Endocrinology Research CenterKevin Knudtson - Iowa Diabetes and Endocrinology Research CenterR.S Bar - University of Iowa
- Resource Type
- Journal article
- Publication Details
- Growth hormone & IGF research, Vol.12(2), pp.91-98
- Publisher
- Elsevier Ltd
- DOI
- 10.1054/ghir.2001.0266
- PMID
- 12175646
- ISSN
- 1096-6374
- eISSN
- 1532-2238
- Language
- English
- Date published
- 04/01/2002
- Academic Unit
- Endocrinology and Metabolism; Internal Medicine; Iowa Institute of Human Genetics
- Record Identifier
- 9984627207602771
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