Journal article
Studies on microinjection of mouse spermatozoa into rabbit oocytes
Shi yan sheng wu xue bao, Vol.32(2), pp.101-111
06/01/1999
PMID: 12548774
Abstract
The methods of intracytoplasmic sperm injection (ICSI) and subzonal injection (SUZI) were used to study heterologous fertilization and embryonic development between the mouse and the rabbit. Results were as follows: 1. The mouse sperm nuclei decondensed and formed pronuclei following microinjection into cytoplasm and perivitelline space (PVS) of rabbit oocytes; 2. The hybrid embryos developed to the stage of 8-cell when cultured in vitro; 3. The karyotype analysis showed a normal complement of rabbit oocyte and mouse sperm chromosomes in the 4-cell hybrid embryos; 4. The ultrastructure of 4-cell hybrid embryos was similar to that of normal 4-cell rabbit embryos; 5. The fertilization rate (32.4%) and cleavage rate (22.2%) when 5-10 mouse spermatozoa were injected were higher than those of injection of a single spermatozoon into PVS of the rabbit oocyte, but the difference was not significant (P > 0.05). The fertilization rate (42.3%) and cleavage rate (30.8%) in rabbit oocytes in vitro matured for 11-12 h w
Details
- Title: Subtitle
- Studies on microinjection of mouse spermatozoa into rabbit oocytes
- Creators
- Z Y LiJ H TanQ ZhouX S SunG P LiZ H Liu
- Resource Type
- Journal article
- Publication Details
- Shi yan sheng wu xue bao, Vol.32(2), pp.101-111
- PMID
- 12548774
- ISSN
- 0001-5334
- Language
- Chinese
- Date published
- 06/01/1999
- Academic Unit
- Anatomy and Cell Biology
- Record Identifier
- 9984284449602771
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