Journal article
Studies on the mechanism of T-cell-mediated lysis: XIII. Lectin-dependent T-cell-mediated cytotoxicity is supported by Con A-coupled Sepharose beads
Cellular immunology, Vol.59(2), pp.411-418
1981
DOI: 10.1016/0008-8749(81)90420-2
PMID: 6974603
Abstract
In an attempt to characterize the “activation” signal delivered by concanavalin A (Con A) in lectin-dependent T-cell-mediated cytotoxicity (LDCC), we attempted to determine whether there was a need for the intracellular localization of lectin within the effector cell. To preclude such internalization, Con A was insolubilized by coupling to Sepharose and was then tested for its ability to support LDCC. We found that all the preparations of Con A-Sepharose tested were effective in supporting cytolysis, suggesting that the “activation” signal for lysis could be delivered solely at the level of the effector cell membrane. Furthermore, in contrast to previous reports, we found that insolubilized Con A was mitogenic for T-cell populations.
Details
- Title: Subtitle
- Studies on the mechanism of T-cell-mediated lysis: XIII. Lectin-dependent T-cell-mediated cytotoxicity is supported by Con A-coupled Sepharose beads
- Creators
- Zuhair K BallasWilliam R GreenChristopher S Henney
- Resource Type
- Journal article
- Publication Details
- Cellular immunology, Vol.59(2), pp.411-418
- Publisher
- Elsevier Inc
- DOI
- 10.1016/0008-8749(81)90420-2
- PMID
- 6974603
- ISSN
- 0008-8749
- eISSN
- 1090-2163
- Language
- English
- Date published
- 1981
- Academic Unit
- Immunology; Internal Medicine
- Record Identifier
- 9984094730402771
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