Journal article
Study of structurally defined oligosaccharide substrates of heparin and heparan monosulfate lyases
Carbohydrate research, Vol.190(2), pp.219-233
1989
DOI: 10.1016/0008-6215(89)84127-8
PMID: 2805009
Abstract
The rapid preparation of multimilligram quantities of five heparin-derived oligosaccharides (
1–5) is described. These oligosaccharides are the final products obtained from the action of heparin lyase (heparinase, E.C. 4.2.2.7) at its primary sites in the heparin polymer. Five oligosaccharides comprise from 75–85 wt% of commercial porcine mucosal heparins and are recovered in good yield and high purity. Four of these five oligosaccharides were further acted upon at much lower rates by prolonged treatment with heparin lyase or heparan monosulfate lyase (heparitinase, E.C. 4.2.2.8), revealing the subspecificities of these enzymes. These oligosaccharides were used as defined substrates for heparin lyase and heparan monosulfate lyase and their kinetic constants were obtained. Potential applications for these oligosaccharides include their use as defined substrates for purification of heparin monosulfate lyases, and for establishing the catalytic purity of enzyme preparations.
Details
- Title: Subtitle
- Study of structurally defined oligosaccharide substrates of heparin and heparan monosulfate lyases
- Creators
- Kevin G RiceRobert J Linhardt
- Resource Type
- Journal article
- Publication Details
- Carbohydrate research, Vol.190(2), pp.219-233
- DOI
- 10.1016/0008-6215(89)84127-8
- PMID
- 2805009
- NLM abbreviation
- Carbohydr Res
- ISSN
- 0008-6215
- eISSN
- 1873-426X
- Publisher
- Elsevier Ltd
- Language
- English
- Date published
- 1989
- Academic Unit
- Pharmaceutical Sciences and Experimental Therapeutics; Craniofacial Anomalies Research Center; Medicinal and Natural Products Chemistry
- Record Identifier
- 9984065699802771
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