Journal article
Study of tensiometric properties, microbiological and collagen content in nile tilapia skin submitted to different sterilization methods
Cell and tissue banking, Vol.19(3), pp.373-382
09/01/2018
DOI: 10.1007/s10561-017-9681-y
PMID: 29380095
Abstract
Tissue bioengineering development is a global concern and different materials are studied and created to be safe, effective and with low cost. Nile Tilapia skin had shown its biological potential as covers for the burn wound. This study evaluates the tilapia skin histological, collagen properties and tensiometric resistance, after treatment by different sterilization methods. Tilapia skin samples were submitted to two sterilization processes: (1) chemical, which consisted in two 2% chlorhexidin baths, followed by sequential baths in increasing glycerol concentrations; and (2) radiation, when glycerolized skin samples were submitted to gamma radiation at 25, 30 and 50 kGy. Microscopic analyzes were performed through Haematoxylin-eosin and Picrosirius Red under polarized light. For tensiometric analysis, traction tests were performed. Glycerol treated skin presented a discrete collagen fibers disorganization within the deep dermis, while irradiated skin did not show any additional change. Throughout the steps of chemical sterilization, there was a higher proportion of collagen with red/yellow birefringence (type I) in the skin samples up to the first bath in chlorhexidin, when compared to samples after the first two glycerol baths (P < 0.005). However, there was no difference in relation to total collagen between groups. In irradiated skin, there was a larger total collagen preservation when using until 30 kGy (P < 0.005). Tensiometric evaluation did not show significant differences in relation to maximum load in the groups studied. We concluded that chemical and radiation (25 and 30 kGy) are efficient methods to sterilize Nile Tilapia skin without altering its microscopic or tensiometric characteristics.
Details
- Title: Subtitle
- Study of tensiometric properties, microbiological and collagen content in nile tilapia skin submitted to different sterilization methods
- Creators
- Ana Paula Negreiros Nunes Alves - Univ Fed Ceara, Nursing Dent & Pharm Sch, 07,17th St, BR-61925430 Fortaleza, Ceara, BrazilEdmar Maciel Lima Junior - Inst Burning Support, Fortaleza, Ceara, BrazilNelson Sarto Piccolo - Fundação de Apoio a Pesquisa do Estado de GoiásMarcelo Jose Borges de Miranda - Hosp Sao Marcos Rede Dor, Recife, PE, BrazilMaria Elisa Quezado Lima Verde - Univ Fed Ceara, Nursing Dent & Pharm Sch, 07,17th St, BR-61925430 Fortaleza, Ceara, BrazilAntonio Ernando Carlos Ferreira Junior - Univ Fed Ceara, Nursing Dent & Pharm Sch, 07,17th St, BR-61925430 Fortaleza, Ceara, BrazilPaulo Goberlanio de Barros Silva - Universidade Federal do CearáVictor Pinheiro Feitosa - Universidade Federal do CearáTereza Jesus Pinheiro Gomes de Bandeira - Univ Fed Ceara, Microbiologist Postgrad Program Med Microbiol, Fortaleza, Ceara, BrazilMonica Beatriz Mathor - Universidade de São PauloManoel Odorico de Moraes - Hospital Geral de Fortaleza
- Resource Type
- Journal article
- Publication Details
- Cell and tissue banking, Vol.19(3), pp.373-382
- DOI
- 10.1007/s10561-017-9681-y
- PMID
- 29380095
- NLM abbreviation
- Cell Tissue Bank
- ISSN
- 1389-9333
- eISSN
- 1573-6814
- Publisher
- Springer Nature
- Number of pages
- 10
- Language
- English
- Date published
- 09/01/2018
- Academic Unit
- Operative Dentistry
- Record Identifier
- 9984656617302771
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