Journal article
Study of the role of the htrB gene in Salmonella typhimurium virulence
Infection and immunity, Vol.65(11), pp.4778-4783
11/1997
DOI: 10.1128/IAI.65.11.4778-4783.1997
PMCID: PMC175685
PMID: 9353064
Abstract
We have undertaken a study to investigate the contribution of the htrB gene to the virulence of pathogenic Salmonella typhimurium. An htrB::mini-Tn10 mutation from Escherichia coli was transferred by transduction to the mouse-virulent strain S. typhimurium SL1344 to create an htrB mutant. The S. typhimurium htrB mutant was inoculated into mice and found to be severely limited in its ability to colonize organs of the lymphatic system and to cause systemic disease in mice. A variety of experiments were performed to determine the possible reasons for this loss of virulence. Serum killing assays revealed that the S. typhimurium htrB mutant was as resistant to killing by complement as the wild-type strain. However, macrophage survival assays revealed that the S. typhimurium htrB mutant was more sensitive to the intracellular environment of murine macrophages than the wild-type strain. In addition, the bioactivity of the lipopolysaccharide (LPS) of the htrB mutant was reduced compared to that of the LPS from the parent strain as measured by both a Limulus amoebocyte lysate endotoxin quantitation assay and a tumor necrosis factor alpha bioassay. These results indicate that the htrB gene plays a role in the virulence of S. typhimurium.
Details
- Title: Subtitle
- Study of the role of the htrB gene in Salmonella typhimurium virulence
- Creators
- Bradley D Jones - Department of Microbiology, University of Iowa College of Medicine, Iowa City 52242-1109, USA. bjones@blue.weeg.uiowa.eduWade A Nichols - Department of Microbiology, University of Iowa College of Medicine, Iowa City 52242-1109, USA. bjones@blue.weeg.uiowa.eduBradford W Gibson - Department of Microbiology, University of Iowa College of Medicine, Iowa City 52242-1109, USA. bjones@blue.weeg.uiowa.eduMelvin G Sunshine - Department of Microbiology, University of Iowa College of Medicine, Iowa City 52242-1109, USA. bjones@blue.weeg.uiowa.eduMichael A Apicella - Department of Microbiology, University of Iowa College of Medicine, Iowa City 52242-1109, USA. bjones@blue.weeg.uiowa.edu
- Resource Type
- Journal article
- Publication Details
- Infection and immunity, Vol.65(11), pp.4778-4783
- DOI
- 10.1128/IAI.65.11.4778-4783.1997
- PMID
- 9353064
- PMCID
- PMC175685
- ISSN
- 0019-9567
- eISSN
- 1098-5522
- Language
- English
- Date published
- 11/1997
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984083298902771
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