Journal article
Subacute exposure to N-ethyl perfluorooctanesulfonamidoethanol results in the formation of perfluorooctanesulfonate and alters superoxide dismutase activity in female rats
Archives of toxicology, Vol.83(10), pp.909-924
10/2009
DOI: 10.1007/s00204-009-0450-y
PMCID: PMC2755535
PMID: 19544052
Abstract
Perfluorooctanesulfonamides, such as N-ethyl perfluorooctanesulfonamidoethanol (N-EtFOSE), are large scale industrial chemicals but their disposition and toxicity are poorly understood despite significant human exposure. The hypothesis that subacute exposure to N-EtFOSE, a weak peroxisome proliferator, causes a redox imbalance in vivo was tested using the known peroxisome proliferator, ciprofibrate, as a positive control. Female Sprague-Dawley rats were treated orally with N-EtFOSE, ciprofibrate or corn oil (vehicle) for 21 days, and levels of N-EtFOSE and its metabolites as well as markers of peroxisome proliferation and oxidative stress were assessed in serum, liver and/or uterus. The N-EtFOSE metabolite profile in liver and serum was in good agreement with reported in vitro biotransformation pathways in rats and the metabolite levels decreasing in the order perfluorooctanesulfonate >> perfluorooctanesulfonamide ~ N-ethyl perfluorooctanesulfonamidoacetate >> perfluorooctanesulfonamidoethanol approximately N-EtFOSE. Although N-EtFOSE treatment significantly decreased the growth rate, increased relative liver weight and activity of superoxide dismutases (SOD) in liver and uterus (total SOD, CuZnSOD and MnSOD), a metabolic study revealed no differences in the metabolome in serum from N-EtFOSE-treated and control animals. Ciprofibrate treatment increased liver weight and peroxisomal acyl Co-A oxidase activity in the liver and altered antioxidant enzyme activities in the uterus and liver. According to NMR metabolomic studies, ciprofibrate treated animals had altered serum lipid profiles compared to N-EtFOSE-treated and control animals, whereas putative markers of peroxisome proliferation in serum were not affected. Overall, this study demonstrates the biotransformation of N-EtFOSE to PFOS in rats that is accompanied by N-EtFOSE-induced alterations in antioxidant enzyme activity.
Details
- Title: Subtitle
- Subacute exposure to N-ethyl perfluorooctanesulfonamidoethanol results in the formation of perfluorooctanesulfonate and alters superoxide dismutase activity in female rats
- Creators
- Wei Xie - Department of Occupational and Environmental Health, College of Public Health, The University of Iowa, 100 Oakdale Campus, #221 IREH, Iowa City, IA 52242, USAQian WuIzabela Kania-KorwelJob C TharappelSanjay TeluMitchell C ColemanHoward P GlauertKurunthachalam KannanS V S MariappanDouglas R SpitzJamie WeydertHans-Joachim Lehmler
- Resource Type
- Journal article
- Publication Details
- Archives of toxicology, Vol.83(10), pp.909-924
- DOI
- 10.1007/s00204-009-0450-y
- PMID
- 19544052
- PMCID
- PMC2755535
- NLM abbreviation
- Arch Toxicol
- ISSN
- 0340-5761
- eISSN
- 1432-0738
- Publisher
- Germany
- Grant note
- P30 ES005605 / NIEHS NIH HHS P30 ES005605-17 / NIEHS NIH HHS P42 ES013661 / NIEHS NIH HHS P42 ES013661-02 / NIEHS NIH HHS ES012475 / NIEHS NIH HHS P30 CA086862 / NCI NIH HHS K25 ES012475 / NIEHS NIH HHS ES013661 / NIEHS NIH HHS P30 ES005605-19 / NIEHS NIH HHS P42 ES013661-03 / NIEHS NIH HHS K25 ES012475-04 / NIEHS NIH HHS K25 ES012475-05 / NIEHS NIH HHS P42 ES013661-04 / NIEHS NIH HHS P30 ES005605-18 / NIEHS NIH HHS P30-CA086862 / NCI NIH HHS P30 CA086862-069012 / NCI NIH HHS ES05605 / NIEHS NIH HHS
- Language
- English
- Date published
- 10/2009
- Academic Unit
- Occupational and Environmental Health; Pathology; Iowa Neuroscience Institute; Orthopedics and Rehabilitation; Radiation Oncology; Iowa Superfund Research Program
- Record Identifier
- 9984000924202771
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