Journal article
Subcellular localization of histone messenger RNAs on cytoskeleton-associated free polysomes in HeLa S3 cells
Journal of cellular physiology, Vol.125(2), pp.345-353
11/1985
DOI: 10.1002/jcp.1041250225
PMID: 3877061
Abstract
We have examined the subcellular distribution of histone mRNA-containing polysomes in HeLa S3 cells to assess the possible relationship between localization of histone mRNAs and the regulation of cellular histone mRNA levels. The distribution of histone mRNAs on free and membrane bound polysomes was examined as well as the association of histone mRNA-containing polysomes with the cytoskeleton. The subcellular localization of histone mRNAs was compared with that of HLA-B7 mRNAs which encode a cell surface antigen. Histone mRNAs were localized predominantly on the free polysomes, whereas the HLA-B7 mRNA was found almost exclusively on membrane bound polysomes. However, both species of mRNA were found associated with the cytoskeleton. Interruption of DNA synthesis by hydroxyurea treatment resulted in a rapid and selective destabilization of histone mRNAs in each subcellular fraction; in contrast, the stability of HLA-B7 mRNA appeared unaffected. The results presented confirm that histone mRNAs are predominantly located on non-membrane bound polysomes and suggest that these polysomes are associated with the cytoskeletal framework.
Details
- Title: Subtitle
- Subcellular localization of histone messenger RNAs on cytoskeleton-associated free polysomes in HeLa S3 cells
- Creators
- Gerard Zambetti - Department of Immunology and Medical Microbiology University of Florida, Gainesville, Florida 32610Janet Stein - Department of Immunology and Medical Microbiology University of Florida, Gainesville, Florida 32610Gary Stein - Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, Florida 32610Warren Schmidt - Department of Pathology, Vanderbilt University, Nashville, Tennessee
- Resource Type
- Journal article
- Publication Details
- Journal of cellular physiology, Vol.125(2), pp.345-353
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company
- DOI
- 10.1002/jcp.1041250225
- PMID
- 3877061
- ISSN
- 0021-9541
- eISSN
- 1097-4652
- Number of pages
- 9
- Language
- English
- Date published
- 11/1985
- Academic Unit
- Gastroenterology and Hepatology; Internal Medicine
- Record Identifier
- 9984094734502771
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