Subcutaneous tissue response and osteogenic performance of calcium phosphate nanoparticle-enriched hydrogels in the tibial medullary cavity of guinea pigs

Matilde Bongio; Jeroen J.J. van den Beucken; M. Reza Nejadnik; Zeinab Tahmasebi Birgani; Pamela Habibovic; Lucas A. Kinard; F. Kurtis Kasper; Antonios G. Mikos; Sander C.G. Leeuwenburgh; John A. Jansen

doi:10.1016/j.actbio.2012.10.026

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Subcutaneous tissue response and osteogenic performance of calcium phosphate nanoparticle-enriched hydrogels in the tibial medullary cavity of guinea pigs

Journal article

Peer reviewed

Subcutaneous tissue response and osteogenic performance of calcium phosphate nanoparticle-enriched hydrogels in the tibial medullary cavity of guinea pigs

Matilde Bongio, Jeroen J.J. van den Beucken, M. Reza Nejadnik, Zeinab Tahmasebi Birgani, Pamela Habibovic, Lucas A. Kinard, F. Kurtis Kasper, Antonios G. Mikos, Sander C.G. Leeuwenburgh and John A. Jansen

Acta biomaterialia, Vol.9(3), pp.5464-5474

03/01/2013

DOI: 10.1016/j.actbio.2012.10.026

PMID: 23107797

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Abstract

In the current study, oligo(poly(ethylene glycol) fumarate) (OPF)-based hydrogels were tested for the first time as injectable bone substitute materials. The primary feature of the material design was the incorporation of calcium phosphate (CaP) nanoparticles within the polymeric matrix in order to compare the soft tissue response and bone-forming capacity of plain OPF hydrogels with CaP-enriched OPF hydrogel composites. To that end, pre-set scaffolds were implanted subcutaneously, whereas flowable polymeric precursor solutions were injected in a tibial ablation model in guinea pigs. After 8weeks of implantation, histological and histomorphometrical evaluation of the subcutaneous scaffolds confirmed the biocompatibility of both types of hydrogels. Nevertheless, OPF hydrogels presented a loose structure, massive cellular infiltration and extensive material degradation compared to OPF–CaP hydrogels that were more compact. Microcomputed tomography and histological and histomorphometrical analyses showed comparable amounts of new trabecular bone in all tibias and some material remnants in the medial and distal regions. Particularly, highly calcified areas were observed in the distal region of OPF–CaP-treated tibias, which indicate a heterogeneous distribution of the mineral phase throughout the hydrogel matrix. This phenomenon can be attributed to either hindered gelation under highly perfused in vivo conditions or a faster degradation rate of the polymeric hydrogel matrix compared to the nanostructured mineral phase, resulting in loss of entrapment of the CaP nanoparticles and subsequent sedimentation.

Animal model

Bone formation

Calcium phosphate nanoparticles

Hydrogels

Injectable

Details

Title: Subtitle: Subcutaneous tissue response and osteogenic performance of calcium phosphate nanoparticle-enriched hydrogels in the tibial medullary cavity of guinea pigs
Creators: Matilde Bongio - Radboud University Medical Center
Jeroen J.J. van den Beucken - Radboud University Medical Center
M. Reza Nejadnik - Radboud University Medical Center
Zeinab Tahmasebi Birgani - University of Twente
Pamela Habibovic - University of Twente
Lucas A. Kinard - Rice University
F. Kurtis Kasper - Rice University
Antonios G. Mikos - Rice University
Sander C.G. Leeuwenburgh - Radboud University Medical Center
John A. Jansen - Radboud University Medical Center
Resource Type: Journal article
Publication Details: Acta biomaterialia, Vol.9(3), pp.5464-5474
Publisher: Elsevier Ltd
DOI: 10.1016/j.actbio.2012.10.026
PMID: 23107797
ISSN: 1742-7061
eISSN: 1878-7568
Language: English
Date published: 03/01/2013
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Pharmaceutical Sciences and Experimental Therapeutics
Record Identifier: 9984420928102771

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