Subfornical organ: Effects of salt loading and water deprivation on in vitro radioamino acid incorporation into individual proteins

Donna M. Santer; William E. Heydorn; G.Joseph Creed; David C. Klein; David M. Jacobowitz

doi:10.1016/0006-8993(86)91463-0

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Subfornical organ: Effects of salt loading and water deprivation on in vitro radioamino acid incorporation into individual proteins

Journal article

Peer reviewed

Subfornical organ: Effects of salt loading and water deprivation on in vitro radioamino acid incorporation into individual proteins

Donna M. Santer, William E. Heydorn, G.Joseph Creed, David C. Klein and David M. Jacobowitz

Brain research, Vol.372(1), pp.107-114

04/30/1986

DOI: 10.1016/0006-8993(86)91463-0

PMID: 3708350

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Abstract

The subfornical organ of the brain has a role in the regulation of fluid balance in higher animals. In this study the effects of salt loading and water deprivation on specific proteins in this organ were investigated. For 4 days, 3 groups of rats were given an appropriate fluid diet (control, 2% NaCl and water deprived), with all groups having free access to food. Animals were killed by decapitation, and the subfornical organ was quickly dissected out and incubated for 6 h in a medium containing [ 35S]methionine and [ 35S]cysteine. Proteins from these organs were then separated by two-dimensional electrophoresis, and the resulting autofluorographs were analyzed by scanning densitometry. The results show that the incorporation of labeled amino acids into 8 proteins was changed due to the experimental manipulations.

autofluorography

computerized scanning densitometry

fluid homeostasis

radioamino acid incorporation into protein

salt loading

subfornical organ

two-dimensional gel electrophoresis

water deprivation

Details

Title: Subtitle: Subfornical organ: Effects of salt loading and water deprivation on in vitro radioamino acid incorporation into individual proteins
Creators: Donna M. Santer - National Institute of Mental Health
William E. Heydorn - United States Food and Drug Administration
G.Joseph Creed - Laboratory of Clinical Science, National Institute of Mental Health, Bethesda, MD 20892, U.S.A
David C. Klein - Eunice Kennedy Shriver National Institute of Child Health and Human Development
David M. Jacobowitz - National Institute of Mental Health
Resource Type: Journal article
Publication Details: Brain research, Vol.372(1), pp.107-114
Publisher: Elsevier B.V
DOI: 10.1016/0006-8993(86)91463-0
PMID: 3708350
ISSN: 0006-8993
eISSN: 1872-6240
Language: English
Date published: 04/30/1986
Academic Unit: Stead Family Department of Pediatrics; General Pediatrics and Adolescent Medicine
Record Identifier: 9984385853302771

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