Sublingual Vaccination Induces Mucosal and Systemic Adaptive Immunity for Protection against Lung Tumor Challenge

Shailbala Singh; Guojun Yang; Kimberly S Schluns; Scott M Anthony; K. Jagannadha Sastry

doi:10.1371/journal.pone.0090001

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Sublingual Vaccination Induces Mucosal and Systemic Adaptive Immunity for Protection against Lung Tumor Challenge

Journal article

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Sublingual Vaccination Induces Mucosal and Systemic Adaptive Immunity for Protection against Lung Tumor Challenge

Shailbala Singh, Guojun Yang, Kimberly S Schluns, Scott M Anthony and K. Jagannadha Sastry

PloS one, Vol.9(3), pp.e90001-e90001

03/05/2014

DOI: 10.1371/journal.pone.0090001

PMCID: PMC3943861

PMID: 24599269

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Abstract

Sublingual route offers a safer and more practical approach for delivering vaccines relative to other systemic and mucosal immunization strategies. Here we present evidence demonstrating protection against ovalbumin expressing B16 (B16-OVA) metastatic melanoma lung tumor formation by sublingual vaccination with the model tumor antigen OVA plus synthetic glycolipid alpha-galactosylceramide (aGalCer) for harnessing the adjuvant potential of natural killer T (NKT) cells, which effectively bridge innate and adaptive arms of the immune system. The protective efficacy of immunization with OVA plus aGalCer was antigen-specific as immunized mice challenged with parental B16 tumors lacking OVA expression were not protected. Multiple sublingual immunizations in the presence, but not in the absence of aGalCer, resulted in repeated activation of NKT cells in the draining lymph nodes, spleens, and lungs of immunized animals concurrent with progressively increasing OVA-specific CD8 + T cell responses as well as serum IgG and vaginal IgA levels. Furthermore, sublingual administration of the antigen only in the presence of the aGalCer adjuvant effectively boosted the OVA-specific immune responses. These results support potential clinical utility of sublingual route of vaccination with aGalCer-for prevention of pulmonary metastases.

Biology

Details

Title: Subtitle: Sublingual Vaccination Induces Mucosal and Systemic Adaptive Immunity for Protection against Lung Tumor Challenge
Creators: Shailbala Singh - The University of Texas MD Anderson Cancer Center
Guojun Yang - The University of Texas MD Anderson Cancer Center
Kimberly S Schluns - The University of Texas at Austin
Scott M Anthony - The University of Texas at Austin
K. Jagannadha Sastry - The University of Texas at Austin
Resource Type: Journal article
Publication Details: PloS one, Vol.9(3), pp.e90001-e90001
DOI: 10.1371/journal.pone.0090001
PMID: 24599269
PMCID: PMC3943861
NLM abbreviation: PLoS One
ISSN: 1932-6203
eISSN: 1932-6203
Publisher: Public Library of Science
Alternative title: Sublingual Vaccination Protects against Lung Tumor
Language: English
Date published: 03/05/2014
Academic Unit: Pathology
Record Identifier: 9984185279202771

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