Journal article
Substrate deformations induce directed keratinocyte migration
Journal of the Royal Society interface, Vol.15(143), pp.20180133-20180133
06/2018
DOI: 10.1098/rsif.2018.0133
PMCID: PMC6030620
PMID: 29899159
Abstract
Cell migration is an essential part of many (patho)physiological processes, including keratinocyte re-epithelialization of healing wounds. Physical forces and mechanical cues from the wound bed (in addition to biochemical signals) may also play an important role in the healing process. Previously, we explored this possibility and found that polyacrylamide (PA) gel stiffness affected human keratinocyte behaviour and that mechanical deformations in soft (approx. 1.2 kPa) PA gels produced by neighbouring cells appeared to influence the process of
epithelial sheet formation. To clearly demonstrate that keratinocytes do respond to such deformations, we conducted a series of experiments where we observed the response of single keratinocytes to a prescribed local substrate deformation that mimicked a neighbouring cell or evolving multicellular aggregate via a servo-controlled microneedle. We also examined the effect of adding either Y27632 or blebbistatin on cell response. Our results indicate that keratinocytes do sense and respond to mechanical signals comparable to those that originate from substrate deformations imposed by neighbouring cells, a finding that could have important implications for the process of keratinocyte re-epithelialization that takes place during wound healing. Furthermore, the Rho/ROCK pathway and the engagement of NM II are both essential to substrate deformation-directed keratinocyte migration.
Details
- Title: Subtitle
- Substrate deformations induce directed keratinocyte migration
- Creators
- Hoda Zarkoob - Department of Biomedical Engineering, College of Engineering, University of Iowa, Iowa City, IA, USASathivel Chinnathambi - Department of Biomedical Engineering, College of Engineering, University of Iowa, Iowa City, IA, USAJohn C Selby - Department of Dermatology, Carver College of Medicine, University of Iowa, Iowa City, IA, USA john-selby@uiowa.eduEdward A Sander - Department of Biomedical Engineering, College of Engineering, University of Iowa, Iowa City, IA, USA edward-sander@uiowa.edu
- Resource Type
- Journal article
- Publication Details
- Journal of the Royal Society interface, Vol.15(143), pp.20180133-20180133
- DOI
- 10.1098/rsif.2018.0133
- PMID
- 29899159
- PMCID
- PMC6030620
- NLM abbreviation
- J R Soc Interface
- ISSN
- 1742-5689
- eISSN
- 1742-5662
- Publisher
- England
- Grant note
- DOI: 10.13039/100000147, name: Division of Civil, Mechanical and Manufacturing Innovation, award: CAREER 1452728; DOI: 10.13039/100001582, name: Dermatology Foundation
- Language
- English
- Date published
- 06/2018
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Dermatology; Orthopedics and Rehabilitation; Craniofacial Anomalies Research Center; Chemical and Biochemical Engineering
- Record Identifier
- 9984025685402771
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