Journal article
Subunit identification and reconstitution of the N-type Ca2+ channel complex purified from brain
Science (American Association for the Advancement of Science), Vol.261(5120), pp.486-489
1993
DOI: 10.1126/science.8392754
PMID: 8392754
Abstract
Calcium channels play an important role in regulating various neuronal processes, including synaptic transmission and cellular plasticity. The N-type calcium channels, which are sensitive to omega-conotoxin, are involved in the control of transmitter release from neurons. A functional N-type calcium channel complex was purified from rabbit brain. The channel consists of a 230-kilodalton subunit (alpha 1B) that is tightly associated with a 160-kilodalton subunit (alpha 2 delta), a 57-kilodalton subunit (beta 3), and a 95-kilodalton glycoprotein subunit. The complex formed a functional calcium channel with the same pharmacological properties and conductance as those of the native omega-conotoxin-sensitive calcium channel in neurons.
Details
- Title: Subtitle
- Subunit identification and reconstitution of the N-type Ca2+ channel complex purified from brain
- Creators
- D. R WITCHER - Univ. Iowa coll. medicine, Howard Hughes medical inst., dep. physiology biophysics, Iowa City IA 52242, United StatesM DE WAARD - Univ. Iowa coll. medicine, Howard Hughes medical inst., dep. physiology biophysics, Iowa City IA 52242, United StatesJ SAKAMOTO - Univ. Iowa coll. medicine, Howard Hughes medical inst., dep. physiology biophysics, Iowa City IA 52242, United StatesC FRANZINI-ARMSTRONG - Univ. Iowa coll. medicine, Howard Hughes medical inst., dep. physiology biophysics, Iowa City IA 52242, United StatesM PRAGNELL - Univ. Iowa coll. medicine, Howard Hughes medical inst., dep. physiology biophysics, Iowa City IA 52242, United StatesS. D KAHL - Univ. Iowa coll. medicine, Howard Hughes medical inst., dep. physiology biophysics, Iowa City IA 52242, United StatesK. P CAMPBELL - Univ. Iowa coll. medicine, Howard Hughes medical inst., dep. physiology biophysics, Iowa City IA 52242, United States
- Resource Type
- Journal article
- Publication Details
- Science (American Association for the Advancement of Science), Vol.261(5120), pp.486-489
- Publisher
- American Association for the Advancement of Science; Washington, DC
- DOI
- 10.1126/science.8392754
- PMID
- 8392754
- ISSN
- 0036-8075
- eISSN
- 1095-9203
- Language
- English
- Date published
- 1993
- Academic Unit
- Neurology; Molecular Physiology and Biophysics; Iowa Neuroscience Institute
- Record Identifier
- 9984068388102771
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