Successful production of offspring after superovulation and In vitro culture of embryos from domestic ferrets (Mustela putorius furos)

Z. Y LI; Q. S JIANG; Y. L ZHANG; X. M LIU; J. F ENGELHARDT

doi:10.1530/rep.0.1220611

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Successful production of offspring after superovulation and In vitro culture of embryos from domestic ferrets (Mustela putorius furos)

Journal article

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Peer reviewed

Successful production of offspring after superovulation and In vitro culture of embryos from domestic ferrets (Mustela putorius furos)

Z. Y LI, Q. S JIANG, Y. L ZHANG, X. M LIU and J. F ENGELHARDT

Reproduction (Cambridge, England), Vol.122(4), pp.611-618

2001

DOI: 10.1530/rep.0.1220611

PMID: 11818023

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Abstract

In an effort to expand the use of ferrets as models for genetic disease, several experimental parameters that are required for successful genetic manipulation in this species were investigated. Optimum superovulation (19.3 +/- 0.6 oocytes and embryos per female) was achieved after injections of 100 iu equine chorionic gonadotrophin (eCG) and 150 iu human chorionic gonadotrophin (hCG). The ovulation rate achieved by the treatment was more than double that induced by mating. Mating with a male immediately after hCG treatment did not significantly alter the number of oocytes ovulated or the number of embryos present, indicating that mating is not required for superovulation in ferrets. Of embryos harvested at the one-cell stage, 64.5% and 47.1% developed into blastocysts when cultured in vitro in CZB or TCM-199 plus 10% fetal bovine serum (FBS) media, respectively. In contrast, only 17.1% of embryos cultured in vitro in NCSU-23 developed to the blastocyst stage. Both freshly retrieved and in vitro cultured embryos from cinnamon-coloured parents produced live young when transferred at the eight-cell stage into albino, pseudo-pregnant recipients. The percentage of kits delivered relative to embryos transferred was 61% for freshly retrieved embryos and 32% for embryos cultured in vitro. These results demonstrate successful embryo transfer in ferrets and provide a basis for further study of genetic modelling approaches in this species after embryo manipulation.

Fundamental and applied biological sciences. Psychology

Biological and medical sciences

Embryology: invertebrates and vertebrates. Teratology

Early stages. Segmentation. Gastrulation. Neurulation

Details

Title: Subtitle: Successful production of offspring after superovulation and In vitro culture of embryos from domestic ferrets (Mustela putorius furos)
Creators: Z. Y LI - Department of Anatomy and Cell Biology, College of Medicine, The University of Iowa, Iowa City, IA 52242, United States
Q. S JIANG - Department of Anatomy and Cell Biology, College of Medicine, The University of Iowa, Iowa City, IA 52242, United States
Y. L ZHANG - Department of Anatomy and Cell Biology, College of Medicine, The University of Iowa, Iowa City, IA 52242, United States
X. M LIU - Department of Anatomy and Cell Biology, College of Medicine, The University of Iowa, Iowa City, IA 52242, United States
J. F ENGELHARDT - Department of Anatomy and Cell Biology, College of Medicine, The University of Iowa, Iowa City, IA 52242, United States
Resource Type: Journal article
Publication Details: Reproduction (Cambridge, England), Vol.122(4), pp.611-618
Publisher: Portland; Colchester
DOI: 10.1530/rep.0.1220611
PMID: 11818023
ISSN: 1470-1626
eISSN: 1741-7899
Language: English
Date published: 2001
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Anatomy and Cell Biology; Radiation Oncology; Internal Medicine
Record Identifier: 9984025673402771

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