Journal article
Sucrose mimics the light induction of Arabidopsis nitrate reductase gene transcription
Proceedings of the National Academy of Sciences - PNAS, Vol.89(5), pp.1861-1864
03/01/1992
DOI: 10.1073/pnas.89.5.1861
PMCID: PMC48553
PMID: 1542684
Abstract
Nitrate reductase, the first enzyme in nitrate assimilation, is located at the crossroad of two energy-consuming pathways: nitrate assimilation and carbon fixation. Light, which regulates the expression of many higher-plant carbon fixation genes, also regulates nitrate reductase gene expression. Located in the cytosol, nitrate reductase obtains its reductant not from photosynthesis but from carbohydrate catabolism. This relationship prompted us to investigate the indirect role that light might play, via photosynthesis, in the regulation of nitrate reductase gene expression. We show that sucrose can replace light in eliciting an increase of nitrate reductase mRNA accumulation in dark-adapted green Arabidopsis plants. We show further that sucrose alone is sufficient for the full expression of nitrate reductase genes in etiolated Arabidopsis plants. Finally, using a reporter gene, we show that a 2.7-kilobase region of 5' flanking sequence of the nitrate reductase gene is sufficient to confer the light or the sucrose response.
Details
- Title: Subtitle
- Sucrose mimics the light induction of Arabidopsis nitrate reductase gene transcription
- Creators
- Chi-Lien ChengGregoria N AcedoMichael KristensenMark A Conkling
- Resource Type
- Journal article
- Publication Details
- Proceedings of the National Academy of Sciences - PNAS, Vol.89(5), pp.1861-1864
- DOI
- 10.1073/pnas.89.5.1861
- PMID
- 1542684
- PMCID
- PMC48553
- NLM abbreviation
- Proc Natl Acad Sci U S A
- ISSN
- 0027-8424
- eISSN
- 1091-6490
- Publisher
- National Academy of Sciences
- Language
- English
- Date published
- 03/01/1992
- Academic Unit
- Biology
- Record Identifier
- 9984217427102771
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