Sudden death and myocardial lesions after damage to catecholamine neurons of the nucleus tractus solitarii in rat

William T Talman; Deidre Nitschke Dragon; Susan Y Jones; Steven A Moore; Li-Hsien Lin

doi:10.1007/s10571-012-9835-1

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Sudden death and myocardial lesions after damage to catecholamine neurons of the nucleus tractus solitarii in rat

Journal article

Open access

Peer reviewed

Sudden death and myocardial lesions after damage to catecholamine neurons of the nucleus tractus solitarii in rat

William T Talman, Deidre Nitschke Dragon, Susan Y Jones, Steven A Moore and Li-Hsien Lin

Cellular and molecular neurobiology, Vol.32(7), pp.1119-1126

10/2012

DOI: 10.1007/s10571-012-9835-1

PMCID: PMC4363999

PMID: 22484855

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Abstract

Lesions that remove neurons expressing neurokinin-1 (NK1) receptors from the nucleus tractus solitarii (NTS) without removing catecholaminergic neurons lead to loss of baroreflexes, labile arterial pressure, myocardial lesions, and sudden death. Because destruction of NTS catecholaminergic neurons expressing tyrosine hydroxylase (TH) may also cause lability of arterial pressure and loss of baroreflexes, we sought to test the hypothesis that cardiac lesions associated with lability are not dependent on damage to neurons with NK1 receptors but would also occur when TH neurons in NTS are targeted. To rid the NTS of TH neurons we microinjected anti-dopamine β-hydroxylase conjugated to saporin (anti-DBH-SAP, 42 ng/200 nl) into the NTS. After injection of the toxin unilaterally, immunofluorescent staining confirmed that anti-DBH-SAP decreased the number of neurons and fibers that contain TH and DBH in the injected side of the NTS while sparing neuronal elements expressing NK1 receptors. Bilateral injections in eight rats led to significant lability of arterial pressure. For example, on day 8 standard deviation of mean arterial pressure was 16.8 ± 2.5 mmHg when compared with a standard deviation of 7.83 ± 0.33 mmHg in six rats in which phosphate buffered saline (PBS) had been injected bilaterally. Two rats died suddenly at 5 and 8 days after anti-DBH-SAP injection. Seven-treated animals demonstrated microscopic myocardial necrosis as reported in animals with lesions of NTS neurons expressing NK1 receptors. Thus, cardiac and cardiovascular effects of lesions directed toward catecholamine neurons of the NTS are similar to those following damage directed toward NK1 receptor-containing neurons.

Neurons - pathology

Catecholamines - metabolism

Solitary Nucleus - pathology

Rats

Male

Myocardium - pathology

Death, Sudden, Cardiac - etiology

Rats, Sprague-Dawley

Animals

Myocardium - metabolism

Death, Sudden, Cardiac - pathology

Receptors, Neurokinin-1 - biosynthesis

Neurons - metabolism

Solitary Nucleus - metabolism

Details

Title: Subtitle: Sudden death and myocardial lesions after damage to catecholamine neurons of the nucleus tractus solitarii in rat
Creators: William T Talman - Department of Neurology, Carver College of Medicine, University of Iowa and Department of Veterans Affairs Medical Center, Iowa, IA 52242, USA. william-talman@uiowa.edu
Deidre Nitschke Dragon
Susan Y Jones
Steven A Moore
Li-Hsien Lin
Resource Type: Journal article
Publication Details: Cellular and molecular neurobiology, Vol.32(7), pp.1119-1126
DOI: 10.1007/s10571-012-9835-1
PMID: 22484855
PMCID: PMC4363999
NLM abbreviation: Cell Mol Neurobiol
ISSN: 0272-4340
eISSN: 1573-6830
Publisher: United States
Grant note: R01 HL088090 / NHLBI NIH HHS R01 HL 088090 / NHLBI NIH HHS I01 RX000364 / RRD VA
Language: English
Date published: 10/2012
Academic Unit: Neurology; Pathology; Internal Medicine
Record Identifier: 9984047786502771

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