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Sulfate metabolites of 4-monochlorobiphenyl in whole poplar plants
Journal article   Peer reviewed

Sulfate metabolites of 4-monochlorobiphenyl in whole poplar plants

Guangshu Zhai, Hans-Joachim Lehmler and Jerald L Schnoor
Environmental science & technology, Vol.47(1), pp.557-562
01/02/2013
DOI: 10.1021/es303807f
PMCID: PMC3565590
PMID: 23215248

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Abstract

4-Monochlorobiphenyl (PCB3) has been proven to be transformed into hydroxylated metabolites of PCB3 (OH-PCB3s) in whole poplar plants in our previous work. However, hydroxylated metabolites of PCBs, including OH-PCB3s, as the substrates of sulfotransferases have not been studied in many organisms including plants in vivo. Poplar (Populus deltoides × nigra, DN34) was used to investigate the further metabolism from OH-PCB3s to PCB3 sulfates because it is a model plant and one that is frequently utilized in phytoremediation. Results showed poplar plants could metabolize PCB3 into PCB3 sulfates during 25 day exposures. Three sulfate metabolites, including 2'-PCB3 sulfate, 3'-PCB3 sulfate, and 4'-PCB3 sulfate, were identified in poplar roots and their concentrations increased in the roots from day 10 to day 25. The major products were 2'-PCB3 sulfate and 4'-PCB3 sulfate. However, the concentrations of PCB3 sulfates were much lower than those of OH-PCB3s in the roots, suggesting the sequential transformation of these hydroxylated PCB3 metabolites into PCB3 sulfates in whole poplars. In addition, 2'-PCB3 sulfate or 4'-PCB3 sulfate was also found in the bottom wood samples indicating some translocation or metabolism in woody tissue. Results suggested that OH-PCB3s were the substrates of sulfotransferases which catalyzed the formation of PCB3 sulfates in the metabolic pathway of PCB3.
Water Pollutants, Chemical - metabolism Plant Roots - metabolism Sulfates - metabolism Wood Hydroponics Mass Spectrometry Populus - metabolism Chromatography, High Pressure Liquid Biphenyl Compounds - metabolism

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