Journal article
Sulforaphane inhibited expression of hypoxia‐inducible factor‐1α in human tongue squamous cancer cells and prostate cancer cells
International journal of cancer, Vol.123(6), pp.1255-1261
09/15/2008
DOI: 10.1002/ijc.23647
PMID: 18561315
Abstract
Previous studies show that a number of natural compounds from our diet have anticancer effects. Sulforaphane is the most characterized isothiocyanates (ITCs), which are identified in cruciferous vegetables. Sulforaphane is viewed as a conceptually promising agent in cancer prevention. Because of its ability to induce cancer cell apoptosis, it inhibits progression of benign tumors to malignant tumors and interrupts metastasis. However, the effect of sulforaphane on tongue cancer cell proliferation has not yet been reported, and the mechanisms that sulforaphane inhibits cancer development are still unclear. Hypoxia‐inducible factor 1 (HIF‐1) expression is associated with tumorigenesis and angiogenesis. It regulates the expression of many genes including vascular endothelial growth factor (VEGF), inducible nitric oxide synthase, and lactate dehydrogenase A. In our study, we investigated the effects of sulforaphane on expression of hypoxia‐inducible factor‐1α (HIF‐1α), which was overexpressed in many human malignant tumors, human tongue squamous cell carcinoma and prostate cancer DU145 cells. Sulforaphane inhibited hypoxia induced expression of HIF‐1α via inhibiting synthesis of HIF‐1α. Sulforaphane was also found to inhibit hypoxia induced HIF‐1α expression through activating JNK and ERK signaling pathways, but not AKT pathway. Inhibition of HIF‐1α by sulforaphane resulted in decreasing expression of VEGF. Taken together, these results suggest that sulforaphane is an effective chemopreventive compound against tongue cancers and prostate cell angiogenesis in vitro, and that the HIF‐1α target provides a new sight into the mechanisms of sulforaphane's inhibition against tumor cell proliferation. © 2008 Wiley‐Liss, Inc.
Details
- Title: Subtitle
- Sulforaphane inhibited expression of hypoxia‐inducible factor‐1α in human tongue squamous cancer cells and prostate cancer cells
- Creators
- Hua Yao - University of KentuckyHuiming Wang - Zhejiang UniversityZhuo Zhang - University of KentuckyBing‐Hua JiangJia Luo - West Virginia UniversityXianglin Shi - University of Kentucky
- Resource Type
- Journal article
- Publication Details
- International journal of cancer, Vol.123(6), pp.1255-1261
- Publisher
- Wiley Subscription Services, Inc., A Wiley Company
- DOI
- 10.1002/ijc.23647
- PMID
- 18561315
- ISSN
- 0020-7136
- eISSN
- 1097-0215
- Number of pages
- 7
- Grant note
- National Institutes of Health (NIH) (R01 ES015375; R01 CA119028; R01 CA116697)
- Language
- English
- Date published
- 09/15/2008
- Academic Unit
- Pathology
- Record Identifier
- 9984186549402771
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