Superior cervical ganglionectomy induces changes in growth factor expression in the rat retina

Luke A Wiley; Bruce A Berkowitz; Jena J Steinle

doi:10.1167/iovs.05-0656

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Superior cervical ganglionectomy induces changes in growth factor expression in the rat retina

Journal article

Peer reviewed

Superior cervical ganglionectomy induces changes in growth factor expression in the rat retina

Luke A Wiley, Bruce A Berkowitz and Jena J Steinle

Investigative ophthalmology & visual science, Vol.47(1), pp.439-443

01/2006

DOI: 10.1167/iovs.05-0656

PMID: 16384994

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Abstract

To determine whether sympathetic nerves regulate expression of known angiogenic growth factors. Surgical sympathectomy (SNX) was used to remove sympathetic innervation to the eye. Real-time PCR was used to measure steady state mRNA expression of VEGF, VEGFR-2, angiopoietin-1, and Tie2. Western blot analysis was performed to assess protein expression. Blood-retinal barrier (BRB) permeability surface area product (PS) was measured using enhanced MRI on a separate group of control and SNX rats. mRNA of both VEGF and VEGFR-2 decreased significantly at 6 weeks after SNX. VEGF protein expression also decreased significantly. VEGFR-2 protein was unchanged. Both angiopoietin-1 and Tie2 mRNA expression increased significantly after SNX. Immunoblot analysis showed that angiopoietin-1 protein expression coincided with its mRNA expression. Tie2 protein expression was unaffected. Sympathetic denervation did not significantly increase BRB PS. Surgical SNX results in a reduction in VEGF gene and protein expression, while increasing protein expression of Ang-1. These findings suggest that sympathetic nerves regulate the expression of angiogenic growth factors in rat retina.

Blood-Retinal Barrier

Gene Expression Regulation - physiology

Rats

Vascular Endothelial Growth Factor Receptor-2 - metabolism

Vascular Endothelial Growth Factor A - metabolism

Angiopoietin-1 - metabolism

RNA, Messenger - metabolism

Reverse Transcriptase Polymerase Chain Reaction

Vascular Endothelial Growth Factor A - genetics

Rats, Sprague-Dawley

Blotting, Western

Receptor, TIE-2 - genetics

Vascular Endothelial Growth Factor Receptor-2 - genetics

Superior Cervical Ganglion - physiology

Angiopoietin-1 - genetics

Magnetic Resonance Imaging

Animals

Time Factors

Ganglionectomy

Female

Receptor, TIE-2 - metabolism

Details

Title: Subtitle: Superior cervical ganglionectomy induces changes in growth factor expression in the rat retina
Creators: Luke A Wiley - Department of Physiology, Southern Illinois University School of Medicine, Carbondale, Illinois, USA
Bruce A Berkowitz
Jena J Steinle
Resource Type: Journal article
Publication Details: Investigative ophthalmology & visual science, Vol.47(1), pp.439-443
DOI: 10.1167/iovs.05-0656
PMID: 16384994
NLM abbreviation: Invest Ophthalmol Vis Sci
ISSN: 0146-0404
eISSN: 1552-5783
Publisher: United States
Grant note: R01 EY013831 / NEI NIH HHS
Language: English
Date published: 01/2006
Academic Unit: Ophthalmology and Visual Sciences
Record Identifier: 9983980058602771

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