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Superoxide-mediated Actin Response in Post-hypoxic Endothelial Cells
Journal article   Open access   Peer reviewed

Superoxide-mediated Actin Response in Post-hypoxic Endothelial Cells

Lawrence E Crawford, Emily E Milliken, Kaikobad Irani, Jay L Zweier, Lewis C Becker, Thomas M Johnson, N. Tony Eissa, Ronald G Crystal, Toren Finkel and Pascal J Goldschmidt-Clermont
The Journal of biological chemistry, Vol.271(43), pp.26863-26867
10/25/1996
DOI: 10.1074/jbc.271.43.26863
PMID: 8900169
url
https://doi.org/10.1074/jbc.271.43.26863View
Published (Version of record) Open Access

Abstract

The mechanism leading to changes in the superstructure of endothelial cells exposed to ischemia and reperfusion remains uncharacterized. We show that in post-hypoxic endothelial cells, the simple re-addition of oxygen induces a profound reorganization of the actin cytoskeleton. The total filamentous actin pool increases by 41% and translocation of actin filaments to the submembranous network is observed. Concurrent with the actin polymerization, increased tyrosine phosphorylation of endothelial cell substrates is detected on Western blots. Overexpression of superoxide dismutase using replication incompetent adenovirus inhibits the actin and tyrosine phosphorylation responses to reoxygenation. Inhibition of tyrosine kinases with the isoflavone genistein also suppressed the actin polymerization response to reoxygenation, but unlike superoxide dismutase, genistein also induced the collapse of the superstructure of endothelial cells upon reoxygenation. These experiments support the concept that reoxygenation following a period of hypoxia can induce the remodeling of the actin cytoskeleton in endothelial cells. Such a response requires the intact coupling of superoxide producing pathway(s) with tyrosine kinase pathway(s).

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