Journal article
Suppression of Immunoglobulin-Secreting Cells from Human Peripheral Blood by Toxic-Shock—Syndrome Toxin-1
The Journal of infectious diseases, Vol.153(4), pp.772-779
04/1986
DOI: 10.1093/infdis/153.4.772
PMID: 3512737
Abstract
Toxic-shock syndrome toxin-1 (TSST-1), a nonspecific T lymphocyte mitogen, was shown to cause the suppression of immunoglobulin-secreting cells (ISCs) generated in pokeweed mitogen (PWM)-stimulated cultures of human peripheral blood mononuclear cells (PBMs). Greater than 90% suppression of the PWM-stimulated ISC response was achieved when 2 X 10(6) PBMs were cultured in the presence of 1 microgram of TSST-1. The nonspecific suppression induced by the toxin was dose related; amounts of TSST-1 as low as 1.0 ng/2 X 10(6) cultured cells caused significant suppression of the ISC response. TSST-1-induced suppression was due to the activation of a population of cells that secreted a soluble factor that mediated the resulting suppression. PBMs were activated by a 24-hr preincubation of 2 X 10(6) cells in the presence of 1 microgram of TSST-1 followed by repeated washing to remove residual toxin. These cells, when co-cultured with unstimulated PBMs and PWM, suppressed the normal ISC response. The supernatant factor, active at volumes of 10 microliters/2 X 10(6) PBMs, was generated by harvesting the supernatant fluid of TSST-1-activated cells. The results of these in vitro experiments confirm that TSST-1 is an immunosuppressing agent that affects human lymphocyte function and, in such a capacity, may play a role in the development of toxic shock syndrome in humans.
Details
- Title: Subtitle
- Suppression of Immunoglobulin-Secreting Cells from Human Peripheral Blood by Toxic-Shock—Syndrome Toxin-1
- Creators
- Nancy J PoindexterPatrick M Schlievert
- Resource Type
- Journal article
- Publication Details
- The Journal of infectious diseases, Vol.153(4), pp.772-779
- DOI
- 10.1093/infdis/153.4.772
- PMID
- 3512737
- ISSN
- 0022-1899
- eISSN
- 1537-6613
- Language
- English
- Date published
- 04/1986
- Academic Unit
- Microbiology and Immunology; Internal Medicine
- Record Identifier
- 9984001157602771
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