Journal article
Suppression of the Granulocyte Colony-Stimulating Factor Response to Escherichia coli Challenge by Alcohol Intoxication
Alcoholism, clinical and experimental research, Vol.22(8), pp.1740-1745
11/1998
DOI: 10.1111/j.1530-0277.1998.tb03974.x
PMID: 9835289
Abstract
Alcohol's suppressive effects on polymorphonuclear leukocyte (PMN) production and function increases host susceptibility to a wide variety of infections and impairs the ability of these effector cells to seek and destroy invading pathogens. Granulocyte colony-stimulating factor (G-CSF), an important regulator of PMN production and function, is known to be increased in the plasma during infectious episodes. In previous studies we found acute alcohol intoxication to suppress the tumor necrosis factor-α (TNFα) response to in vivo challenges with bacteria or lipopolysaccharide. The present study was initiated to determine the impact of alcohol intoxication on the plasma G-CSF response to Gram-negative infection. For this purpose, rats received an intravenous challenge of Escherichia coli (106 CFU) 30 min after an intraperitoneal injection of ethanol (5.5 g/kg) or an equivalent volume of saline (control). Ethanol-intoxicated rats had a greater 48 hr mortality to live E. coli injection than did unintoxicated animals (45% vs. 8%). Despite an increased bacterial burden in both the lung and liver at 24 hr after initiating E. coli infection in alcohol-intoxicated animals, PMN tissue recruitment, indexed as myeloperoxidase activity, did not differ between control and alcohol-treated rats. Moreover, alcohol suppressed blood PMN phagocytic capacity to a greater extent in animals given alcohol than controls at 5 and 24 hr after initiating infection. In control animals after intravenous E. coli injection, bioactive G-CSF increased in plasma and peaked near 300 ng/ml at 8 hr. In rats pretreated with alcohol, the plasma G-CSF response was markedly suppressed in response to intravenous E. coli (p 0.05). In a second experiment, neutralization of the E. coli-induced plasma TNFα response by pretreatment with anti-TNFα antibody similarly inhibited the plasma G-CSF response. These results support the postulate that alcohol-induced inhibition of TNFα directly contributes to the adverse effects of alcohol on PMN function by suppressing the normal autocrine amplification pathway responsible for G-CSF production.
Details
- Title: Subtitle
- Suppression of the Granulocyte Colony-Stimulating Factor Response to Escherichia coli Challenge by Alcohol Intoxication
- Creators
- Gregory J BagbyPing ZhangDavid A StoltzSteve Nelson
- Resource Type
- Journal article
- Publication Details
- Alcoholism, clinical and experimental research, Vol.22(8), pp.1740-1745
- DOI
- 10.1111/j.1530-0277.1998.tb03974.x
- PMID
- 9835289
- NLM abbreviation
- Alcohol Clin Exp Res
- ISSN
- 0145-6008
- eISSN
- 1530-0277
- Publisher
- Blackwell Publishing Ltd; Oxford, UK
- Number of pages
- 6
- Language
- English
- Date published
- 11/1998
- Academic Unit
- Roy J. Carver Department of Biomedical Engineering; Molecular Physiology and Biophysics; Pulmonary, Critical Care, and Occupational Medicine; Internal Medicine
- Record Identifier
- 9984025323202771
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