Surfactant-associated protein A provides critical immunoprotection in neonatal mice

Caroline L S George; Kelli L Goss; David K Meyerholz; Fred S Lamb; Jeanne M Snyder

doi:10.1128/IAI.01043-07

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Surfactant-associated protein A provides critical immunoprotection in neonatal mice

Journal article

Open access

Peer reviewed

Surfactant-associated protein A provides critical immunoprotection in neonatal mice

Caroline L S George, Kelli L Goss, David K Meyerholz, Fred S Lamb and Jeanne M Snyder

Infection and immunity, Vol.76(1), pp.380-390

01/2008

DOI: 10.1128/IAI.01043-07

PMCID: PMC2223658

PMID: 17967856

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Abstract

The collectins surfactant-associated protein A (SP-A) and SP-D are components of innate immunity that are present before birth. Both proteins bind pathogens and assist in clearing infection. The significance of SP-A and SP-D as components of the neonatal immune system has not been investigated. To determine the role of SP-A and SP-D in neonatal immunity, wild-type, SP-A null, and SP-D null mice were bred in a bacterium-laden environment (corn dust bedding) or in a semisterile environment (cellulose fiber bedding). When reared in the corn dust bedding, SP-A null pups had significant mortality (P < 0.001) compared to both wild-type and SP-D null pups exposed to the same environment. The mortality of the SP-A null pups was associated with significant gastrointestinal tract pathology but little lung pathology. Moribund SP-A null newborn mice exhibited Bacillus sp. and Enterococcus sp. peritonitis. When the mother or newborn produced SP-A, newborn survival was significantly improved (P < 0.05) compared to the results when there was a complete absence of SP-A in both the mother and the pup. Significant sources of SP-A likely to protect a newborn include the neonatal lung and gastrointestinal tract but not the lactating mammary tissue of the mother. Furthermore, exogenous SP-A delivered by mouth to newborn SP-A null pups with SP-A null mothers improved newborn survival in the corn dust environment. Therefore, a lack of SP-D did not affect newborn survival, while SP-A produced by either the mother or the pup or oral exogenous SP-A significantly reduced newborn mortality associated with environmentally induced infection in SP-A null newborns.

Litter Size

Gene Expression

Immunity, Maternally-Acquired

Zea mays

Humans

Male

Bedding and Linens

Pulmonary Surfactant-Associated Protein A - administration & dosage

Pulmonary Surfactant-Associated Protein D

Dust

Pulmonary Surfactant-Associated Protein A - immunology

Animals

Gene Deletion

Female

Mice

Animals, Newborn - immunology

Pulmonary Surfactant-Associated Protein A - genetics

Details

Title: Subtitle: Surfactant-associated protein A provides critical immunoprotection in neonatal mice
Creators: Caroline L S George - Department of Pediatrics, University of Iowa, Carver College of Medicine, Iowa City, Iowa 52242, USA. cgeorge@umn.edu
Kelli L Goss
David K Meyerholz
Fred S Lamb
Jeanne M Snyder
Resource Type: Journal article
Publication Details: Infection and immunity, Vol.76(1), pp.380-390
DOI: 10.1128/IAI.01043-07
PMID: 17967856
PMCID: PMC2223658
NLM abbreviation: Infect Immun
ISSN: 0019-9567
eISSN: 1098-5522
Grant note: P30 ES005605 / NIEHS NIH HHS P30 ES05605 / NIEHS NIH HHS
Language: English
Date published: 01/2008
Academic Unit: Anatomy and Cell Biology; Stead Family Department of Pediatrics; Pathology
Record Identifier: 9984083225002771

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