Survival of Drosophila germline stem cells requires the chromatin-binding protein Barrier-to-autointegration factor

Tingting Duan; S Cole Kitzman; Pamela K Geyer

doi:10.1242/dev.186171

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Survival of Drosophila germline stem cells requires the chromatin-binding protein Barrier-to-autointegration factor

Journal article

Open access

Peer reviewed

Survival of Drosophila germline stem cells requires the chromatin-binding protein Barrier-to-autointegration factor

Tingting Duan, S Cole Kitzman and Pamela K Geyer

Development (Cambridge), Vol.147(9), dev186171

05/13/2020

DOI: 10.1242/dev.186171

PMCID: PMC7240298

PMID: 32345742

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https://doi.org/10.1242/dev.186171View

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Abstract

The nuclear lamina (NL) is an extensive protein network that underlies the inner nuclear envelope. This network includes LAP2-emerin-MAN1 domain (LEM-D) proteins that associate with the chromatin and DNA-binding protein Barrier-to-autointegration factor (BAF). Here, we investigate the partnership between three NL LEM-D proteins and BAF. In most tissues, only Emerin/Otefin is required for NL enrichment of BAF, revealing an unexpected dependence on a single LEM-D protein. Prompted by these observations, we studied BAF contributions in the ovary, a tissue where Emerin/Otefin function is essential. We show that germ cell-specific BAF knockdown causes phenotypes that mirror mutants. Loss of BAF disrupts NL structure, blocks differentiation and promotes germ cell loss, phenotypes that are partially rescued by inactivation of the ATR and Chk2 kinases. These data suggest that, similar to mutants, BAF depletion activates the NL checkpoint that causes germ cell loss. Taken together, our findings provide evidence for a prominent NL partnership between the LEM-D protein Emerin/Otefin and BAF, revealing that BAF functions with this partner in the maintenance of an adult stem cell population.

Animals

Checkpoint Kinase 2 - metabolism

DNA-Binding Proteins - genetics

DNA-Binding Proteins - metabolism

Drosophila

Drosophila Proteins - genetics

Drosophila Proteins - metabolism

Female

Germ Cells - cytology

Germ Cells - metabolism

Male

Nuclear Lamina - metabolism

Nuclear Proteins - genetics

Nuclear Proteins - metabolism

Oogenesis - genetics

Oogenesis - physiology

Details

Title: Subtitle: Survival of Drosophila germline stem cells requires the chromatin-binding protein Barrier-to-autointegration factor
Creators: Tingting Duan - University of Iowa
S Cole Kitzman - University of Iowa
Pamela K Geyer - University of Iowa
Resource Type: Journal article
Publication Details: Development (Cambridge), Vol.147(9), dev186171
DOI: 10.1242/dev.186171
PMID: 32345742
PMCID: PMC7240298
ISSN: 0950-1991
eISSN: 1477-9129
Grant note: S10 RR025439 / NCRR NIH HHS R01 GM087341 / NIGMS NIH HHS
Language: English
Date published: 05/13/2020
Academic Unit: Obstetrics and Gynecology; Biochemistry and Molecular Biology
Record Identifier: 9984288722402771

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