Survivin expression in juvenile-onset recurrent respiratory papillomatosis

David M Poetker; Anthony D Sandler; David L Scott; Richard J H Smith; Nancy M Bauman

doi:10.1177/000348940211101101

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Survivin expression in juvenile-onset recurrent respiratory papillomatosis

Journal article

Peer reviewed

Survivin expression in juvenile-onset recurrent respiratory papillomatosis

David M Poetker, Anthony D Sandler, David L Scott, Richard J H Smith and Nancy M Bauman

Annals of otology, rhinology & laryngology, Vol.111(11), pp.957-961

11/2002

DOI: 10.1177/000348940211101101

PMID: 12450166

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Abstract

Recurrent respiratory papillomatosis (RRP), caused by the human papillomavirus, is characterized by unregulated growth of wartlike neoplasms on laryngeal mucosa. Apoptosis is important in normal cellular homeostasis, and dysregulation of this process is thought to govern the behavior of certain neoplasms. This study evaluates the expression of several pro-apoptotic and anti-apoptotic factors in papillomas of patients with RRP, with a specific interest in survivin, a cell cycle-regulated anti-apoptotic factor. Three anti-apoptotic and 6 pro-apoptotic messenger RNA (mRNA) species were quantified by ribonuclease protection assay in 11 RRP papilloma specimens and 5 normal laryngeal specimens. Anti-apoptotic and pro-apoptotic mRNA ratios were quantified by normalizing to the ribosomal protein L32 and compared between specimens. Protein expression of survivin in tissue samples was also evaluated. The mean (+/- SD) expression of survivin was almost fivefold greater in the RRP papillomas than in normal tissue (14.2% +/- 2.5% versus 3.0% +/- 0.8% of L32, p = .003). The RRP specimens also had greater expression of XIAP, Fas, and p53 than did the normal tissue. Survivin protein was differentially expressed in the papilloma specimens, and was greatest in a papilloma that underwent malignant transformation. Survivin was absent in all normal laryngeal tissue tested. Apoptotic factors in general appear to be upregulated in papillomatous tissue as compared to normal laryngeal tissue and may suggest a higher proliferation rate and cell turnover. Survivin is abundant in papillomas and absent in normal laryngeal tissue. Dysregulation of apoptosis as determined by abnormal expression of anti-apoptotic factors like survivin and XIAP probably favors papilloma growth and survival. Such factors may represent potential targets in the treatment of this disease.

Up-Regulation

Larynx - metabolism

Age Factors

Microtubule-Associated Proteins - genetics

Humans

Gene Expression Regulation, Neoplastic

RNA, Messenger - analysis

Apoptosis - genetics

Luminescent Measurements

RNA, Neoplasm

Neoplasm Proteins

Papilloma - surgery

fas Receptor - genetics

Adult

Child

Antigens, Neoplasm - genetics

Laryngeal Neoplasms - genetics

Papilloma - genetics

Data Interpretation, Statistical

Laryngeal Neoplasms - surgery

Inhibitor of Apoptosis Proteins

Neoplasm Recurrence, Local

Genes, p53 - genetics

Blotting, Western

Autoradiography

Cysteine Proteinase Inhibitors

Ribosomal Proteins

Details

Title: Subtitle: Survivin expression in juvenile-onset recurrent respiratory papillomatosis
Creators: David M Poetker - Department of Otolaryngology-Head and Neck Surgery, The University of Iowa Hospitals and Clinics, Iowa City 52242, USA
Anthony D Sandler
David L Scott
Richard J H Smith
Nancy M Bauman
Resource Type: Journal article
Publication Details: Annals of otology, rhinology & laryngology, Vol.111(11), pp.957-961
DOI: 10.1177/000348940211101101
PMID: 12450166
NLM abbreviation: Ann Otol Rhinol Laryngol
ISSN: 0003-4894
eISSN: 1943-572X
Publisher: United States
Language: English
Date published: 11/2002
Academic Unit: Roy J. Carver Department of Biomedical Engineering; Molecular Physiology and Biophysics; Anatomy and Cell Biology; Stead Family Department of Pediatrics; Iowa Neuroscience Institute; Otolaryngology; Internal Medicine
Record Identifier: 9984006458202771

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