Survivin mRNA is down-regulated during early Xenopus laevis embryogenesis

Cary R Murphy; Jaime L Sabel; Anthony D Sandler; John M Dagle

doi:10.1002/dvdy.10194

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Survivin mRNA is down-regulated during early Xenopus laevis embryogenesis

Journal article

Open access

Peer reviewed

Survivin mRNA is down-regulated during early Xenopus laevis embryogenesis

Cary R Murphy, Jaime L Sabel, Anthony D Sandler and John M Dagle

Developmental dynamics, Vol.225(4), pp.597-601

12/2002

DOI: 10.1002/dvdy.10194

PMID: 12454937

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Abstract

One of the hallmarks of early development is the rapid proliferation of cells immediately after fertilization. Many of the rules that govern cell division in normal somatic cells, such as contact inhibition and apoptosis, seem temporarily suspended in the early embryo. A similar suspension of mechanisms normally regulating cell division occurs in the development of cancer. Survivin, an inhibitor of apoptosis and a positive regulator of progression through the cell cycle, localizes to the mitotic spindle and interacts with several proapoptotic caspases. Survivin protein expression has been studied during the development of the salivary gland in mouse. However, the regulation of survivin during the critical transitions defining oocyte maturation and the early restriction of developmental potential are not easily examined in the mouse. We therefore studied survivin mRNA expression during oogenesis and early embryogenesis in Xenopus laevis. We found that survivin mRNA is present in the earliest stages of Xenopus oocytes and that it accumulates during oogenesis. Progesterone-induced maturation of Xenopus oocytes leads to polyadenylation of the survivin transcript. Survivin mRNA is also present in early Xenopus embryos. After the onset of zygotic transcription, however, the amount of survivin mRNA declines rapidly to undetectable levels. This decrease in survivin mRNA correlates temporally with both the slowing of the cell cycle and the onset of endogenous embryonic apoptosis. With the exception of the ovary, survivin mRNA was undetectable in all adult Xenopus tissues examined.

Amino Acid Sequence

Xenopus laevis - embryology

Inhibitor of Apoptosis Proteins

Microtubule-Associated Proteins - genetics

Oocytes - metabolism

Down-Regulation

Molecular Sequence Data

Microtubule-Associated Proteins - biosynthesis

RNA, Messenger - metabolism

Sequence Homology, Amino Acid

Animals

Time Factors

Xenopus laevis - metabolism

Neoplasm Proteins

Cloning, Molecular

Polyadenylation

Progesterone - metabolism

Details

Title: Subtitle: Survivin mRNA is down-regulated during early Xenopus laevis embryogenesis
Creators: Cary R Murphy - Department of Pediatrics, University of Iowa, Iowa City, Iowa 52242, USA
Jaime L Sabel
Anthony D Sandler
John M Dagle
Resource Type: Journal article
Publication Details: Developmental dynamics, Vol.225(4), pp.597-601
Publisher: United States
DOI: 10.1002/dvdy.10194
PMID: 12454937
ISSN: 1058-8388
eISSN: 1097-0177
Grant note: HD 27748 / NICHD NIH HHS
Language: English
Date published: 12/2002
Academic Unit: Stead Family Department of Pediatrics; Epidemiology; Biochemistry and Molecular Biology; Neonatology
Record Identifier: 9984025262802771

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