Susceptibility of a polycaprolactone-based root canal-filling material to degradation. III. Turbidimetric evaluation of enzymatic hydrolysis

Noriko Hiraishi; Joyce Y Y Yau; Robert J Loushine; Steven R Armstrong; R Norman Weller; Nigel M King; David H Pashley; Franklin R Tay

doi:10.1016/j.joen.2007.05.004

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Susceptibility of a polycaprolactone-based root canal-filling material to degradation. III. Turbidimetric evaluation of enzymatic hydrolysis

Journal article

Peer reviewed

Susceptibility of a polycaprolactone-based root canal-filling material to degradation. III. Turbidimetric evaluation of enzymatic hydrolysis

Noriko Hiraishi, Joyce Y Y Yau, Robert J Loushine, Steven R Armstrong, R Norman Weller, Nigel M King, David H Pashley and Franklin R Tay

Journal of endodontics, Vol.33(8), pp.952-956

08/2007

DOI: 10.1016/j.joen.2007.05.004

PMID: 17878081

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Abstract

The susceptibility of Resilon (Pentron Clinical Technologies, Wallingford, CT) to biotic biodegradation by two hydrolases, lipase PS and cholesterol esterase, was investigated with a turbidimetric approach by measuring the optical density reductions in aqueous emulsions containing dissolved, filtered, surfactant-stabilized polymeric components of Resilon. Polycaprolactone, the major polymeric component of Resilon, was also examined in a similar manner using equivalent or a four-fold increase in enzyme concentration. Optical density time plots were characterized by an initially linear steep reduction in optical density, from which the reaction rates were derived. For both enzymes, the rates of hydrolysis for Resilon were much faster than those of polycaprolactone at 1x or even 4x enzyme concentration. Field-emission scanning electron microscopy of air-dried Resilon and polycaprolactone emulsions revealed the presence of spherical polymer droplets that appeared deformed, pitted, or much reduced in dimensions after enzymatic hydrolysis.

Biodegradation, Environmental

Hydrolysis

Nephelometry and Turbidimetry

Polyesters - chemistry

Microscopy, Electron, Scanning - methods

Emulsions - chemistry

Lipase - chemistry

Sterol Esterase - chemistry

Spectrophotometry, Ultraviolet

Root Canal Filling Materials - chemistry

Details

Title: Subtitle: Susceptibility of a polycaprolactone-based root canal-filling material to degradation. III. Turbidimetric evaluation of enzymatic hydrolysis
Creators: Noriko Hiraishi - Faculty of Dentistry, The University of Hong Kong, and Prince Philip Dental Hospital, Pokfulam, Hong Kong SAR, China
Joyce Y Y Yau
Robert J Loushine
Steven R Armstrong
R Norman Weller
Nigel M King
David H Pashley
Franklin R Tay
Resource Type: Journal article
Publication Details: Journal of endodontics, Vol.33(8), pp.952-956
DOI: 10.1016/j.joen.2007.05.004
PMID: 17878081
NLM abbreviation: J Endod
ISSN: 0099-2399
eISSN: 1878-3554
Publisher: Elsevier Inc; United States
Language: English
Date published: 08/2007
Academic Unit: Operative Dentistry
Record Identifier: 9984065990002771

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