Susceptibility of nontypeable Haemophilus influenzae to human beta-defensins is influenced by lipooligosaccharide acylation

Timothy D Starner; W Edward Swords; Michael A Apicella; Paul B McCray Jr

doi:10.1128/IAI.70.9.5287-5289.2002

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Susceptibility of nontypeable Haemophilus influenzae to human beta-defensins is influenced by lipooligosaccharide acylation

Journal article

Open access

Peer reviewed

Susceptibility of nontypeable Haemophilus influenzae to human beta-defensins is influenced by lipooligosaccharide acylation

Timothy D Starner, W Edward Swords, Michael A Apicella and Paul B McCray Jr

Infection and immunity, Vol.70(9), pp.5287-5289

09/2002

DOI: 10.1128/IAI.70.9.5287-5289.2002

PMCID: PMC128250

PMID: 12183584

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Abstract

Nontypeable Haemophilus influenzae (NTHI) lipooligosaccharide htrB mutants exhibited greater than 45-fold-increased sensitivity to human beta-defensin 2 (HBD-2) compared to the wild type. Complementation by htrB in trans to acylation competence reversed this increased sensitivity. In contrast, NTHI was more susceptible to HBD-3 and showed no changes in sensitivity as a result of lipooligosaccharide mutations in oligosaccharide and lipid A biosynthesis genes.

Mutation

Genes, Bacterial

Haemophilus influenzae - metabolism

Lipid A - genetics

Lipid A - metabolism

Haemophilus influenzae - drug effects

Humans

Bacterial Proteins - genetics

Drug Resistance, Bacterial

Lipopolysaccharides - metabolism

Haemophilus influenzae - genetics

Acyltransferases - metabolism

Acyltransferases - genetics

Genetic Complementation Test

Haemophilus influenzae - immunology

beta-Defensins - pharmacology

Bacterial Proteins - metabolism

Anti-Bacterial Agents - pharmacology

In Vitro Techniques

Acylation

Details

Title: Subtitle: Susceptibility of nontypeable Haemophilus influenzae to human beta-defensins is influenced by lipooligosaccharide acylation
Creators: Timothy D Starner - Department of Pediatrics, The University of Iowa, Iowa City, Iowa 52242, USA
W Edward Swords
Michael A Apicella
Paul B McCray Jr
Resource Type: Journal article
Publication Details: Infection and immunity, Vol.70(9), pp.5287-5289
DOI: 10.1128/IAI.70.9.5287-5289.2002
PMID: 12183584
PMCID: PMC128250
NLM abbreviation: Infect Immun
ISSN: 0019-9567
eISSN: 1098-5522
Grant note: T32 AI007511 / NIAID NIH HHS HL61234 / NHLBI NIH HHS HL07638 / NHLBI NIH HHS R01 AI024616 / NIAID NIH HHS P50 HL061234 / NHLBI NIH HHS R37 AI024616 / NIAID NIH HHS T32 HL007638 / NHLBI NIH HHS N01AI65298 / NIAID NIH HHS R56 AI024616 / NIAID NIH HHS AI65298 / NIAID NIH HHS AI07511 / NIAID NIH HHS AI24616 / NIAID NIH HHS
Language: English
Date published: 09/2002
Academic Unit: Microbiology and Immunology; Pulmonary Medicine; Stead Family Department of Pediatrics; Internal Medicine
Record Identifier: 9984093488002771

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