Journal article
Syk mediates BCR- and CD40-signaling integration during B cell activation
Immunobiology (1979), Vol.216(5), pp.566-570
2011
DOI: 10.1016/j.imbio.2010.09.016
PMID: 21074890
Abstract
CD40 is essential for optimal B cell activation. It has been shown that CD40 stimulation can augment BCR-induced B cell responses, but the molecular mechanism(s) by which CD40 regulates BCR signaling is poorly understood. In this report, we attempted to characterize the signaling synergy between BCR- and CD40-mediated pathways during B cell activation. We found that spleen tyrosine kinase (Syk) is involved in CD40 signaling, and is synergistically activated in B cells in response to BCR/CD40 costimulation. CD40 stimulation alone also activates B cell linker (BLNK), Bruton tyrosine kinase (Btk), and Vav-2 downstream of Syk, and significantly enhances BCR-induced formation of complex consisting of, Vav-2, Btk, BLNK, and phospholipase C-gamma2 (PLC-γ2) leading to activation of extracellular signal-regulated kinase (ERK), p38 mitogen-activated protein kinase, Akt, and NF-κB required for optimal B cell activation. Therefore, our data suggest that CD40 can strengthen BCR-signaling pathway and quantitatively modify BCR signaling during B cell activation.
Details
- Title: Subtitle
- Syk mediates BCR- and CD40-signaling integration during B cell activation
- Creators
- Haiyan Ying - Section of Nephrology, Department of Medicine, The University of Chicago, 5841 S. Maryland Ave., IL 60637, United StatesZhenping Li - Section of Nephrology, Department of Medicine, The University of Chicago, 5841 S. Maryland Ave., IL 60637, United StatesLifen Yang - Section of Nephrology, Department of Medicine, The University of Chicago, 5841 S. Maryland Ave., IL 60637, United StatesJian Zhang - Section of Nephrology, Department of Medicine, The University of Chicago, 5841 S. Maryland Ave., IL 60637, United States
- Resource Type
- Journal article
- Publication Details
- Immunobiology (1979), Vol.216(5), pp.566-570
- Publisher
- Elsevier GmbH
- DOI
- 10.1016/j.imbio.2010.09.016
- PMID
- 21074890
- ISSN
- 0171-2985
- eISSN
- 1878-3279
- Language
- English
- Date published
- 2011
- Academic Unit
- Pathology
- Record Identifier
- 9984047689502771
Metrics
39 Record Views