Synapsis-defective mutants reveal a correlation between chromosome conformation and the mode of double-strand break repair during Caenorhabditis elegans meiosis

Sarit Smolikov; Andreas Eizinger; Allison Hurlburt; Eric Rogers; Anne M Villeneuve; Mónica P Colaiácovo

doi:10.1534/genetics.107.076968

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Synapsis-defective mutants reveal a correlation between chromosome conformation and the mode of double-strand break repair during Caenorhabditis elegans meiosis

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Synapsis-defective mutants reveal a correlation between chromosome conformation and the mode of double-strand break repair during Caenorhabditis elegans meiosis

Sarit Smolikov, Andreas Eizinger, Allison Hurlburt, Eric Rogers, Anne M Villeneuve and Mónica P Colaiácovo

Genetics (Austin), Vol.176(4), pp.2027-2033

08/2007

DOI: 10.1534/genetics.107.076968

PMCID: PMC1950611

PMID: 17565963

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Abstract

SYP-3 is a new structural component of the synaptonemal complex (SC) required for the regulation of chromosome synapsis. Both chromosome morphogenesis and nuclear organization are altered throughout the germlines of syp-3 mutants. Here, our analysis of syp-3 mutants provides insights into the relationship between chromosome conformation and the repair of meiotic double-strand breaks (DSBs). Although crossover recombination is severely reduced in syp-3 mutants, the production of viable offspring accompanied by the disappearance of RAD-51 foci suggests that DSBs are being repaired in these synapsis-defective mutants. Our studies indicate that once interhomolog recombination is impaired, both intersister recombination and nonhomologous end-joining pathways may contribute to repair during germline meiosis. Moreover, our studies suggest that the conformation of chromosomes may influence the mode of DSB repair employed during meiosis.

Caenorhabditis elegans - metabolism

Crossing Over, Genetic

Caenorhabditis elegans - genetics

Genes, Helminth

Male

Chromosome Pairing - genetics

DNA Breaks, Double-Stranded

Synaptonemal Complex - genetics

Meiosis - genetics

Animals

Caenorhabditis elegans - cytology

Recombination, Genetic

DNA Repair

Female

Mutation

Details

Title: Subtitle: Synapsis-defective mutants reveal a correlation between chromosome conformation and the mode of double-strand break repair during Caenorhabditis elegans meiosis
Creators: Sarit Smolikov - Department of Genetics, Harvard Medical School, Boston, MA 02115, USA
Andreas Eizinger
Allison Hurlburt
Eric Rogers
Anne M Villeneuve
Mónica P Colaiácovo
Resource Type: Journal article
Publication Details: Genetics (Austin), Vol.176(4), pp.2027-2033
Publisher: United States
DOI: 10.1534/genetics.107.076968
PMID: 17565963
PMCID: PMC1950611
ISSN: 0016-6731
eISSN: 1943-2631
Grant note: R01 GM053804 / NIGMS NIH HHS R01 GM 072551 / NIGMS NIH HHS R01 GM 53804 / NIGMS NIH HHS R01 GM072551 / NIGMS NIH HHS
Language: English
Date published: 08/2007
Academic Unit: Biology
Record Identifier: 9983992068202771

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